摘要
以有丝分裂原ＣｏｎＡ或ＬＰＳ诱导的小鼠脾细胞增殖为模型，检测从雷公藤中分离的７个环氧二萜内酯化合物体外对Ｔ、Ｂ淋巴细胞的作用和效价。实验结果表明，６个化合物（Ｔ_４、Ｔ_７、Ｔ_８、Ｔ_９、Ｔ_１０、Ｌ_２）和ＴⅡ组份均显著抑制ＣｏｎＡ或ＬＰＳ诱导的淋巴细胞增殖，这些化合物对两者的抑制效价在１０^（－５）～１０^（－９）μｇ／ｍｌ间，Ｔ_Ⅱ组份则分别为３．５及４．２μｇ／ｍｌ。其ＩＣ５０值由小至大依次是Ｔ_１０、Ｔ_４、Ｔ_８、Ｌ_２、Ｔ_７、Ｔ_９和Ｔ_Ⅱ，在剂量─效应坐标图上ＬＰＳ诱导曲线在ＣｏｎＡ诱导曲线的右侧，两曲线基本平行，ＩＣ５０大小次序一致，结果非常接近。另一个化合物Ｔ_（１１）在浓度达５０μｇ／ｍｌ时对两者均无抑制作用，体外定性结果与体内完全一致。提示雷公藤免疫活性化合物在体外对Ｔ、Ｂ细胞增殖的抑制作用是明确的、直接的和无选择性的。
The inhibitory effects and their IC50 values of Tripterygium wilfordii(TW)on T and B lymphocytes were assayed using mice splenocytes induced to proliferate by mitogens ConA and LPS in vitro. The results indicated that 6 compounds(T_4,T_7,T_8,T_9,T_(10),L_2)and component TⅡ had significant inhibitory effects on the proliferation of lymphocytes in response to ConA and LPS.The inhibitory potencies of these compounds were in the range of 10-5～ 10-9μg/ml for both T and B cells, while those of TⅡ were 3. 5 and 4.2μg/ml, respectively. The IC50 values, from low to high, were in the following order:T_(10),T_4,T_8,L_2,T_7, T_9 and TⅡ,The dose-effect curves for LPS were to the right of ConA curves, though both were nearly parallel. The relative order of the IC_(50_ values determined from ConA and LPS curves were also coincident.Another compound,T_(11), showed no suppressive effect on the proliferation of T and B lymphocytes induced by either mitogen, even when its concentration reached 50μg/ml. The results suggest that the suppressive effects of the componends on the proliferation of T and B lymphocytes in vitro were direct and non-selective.
出处
《中国医学科学院学报》
CSCD
北大核心
1994年第1期24-28,共5页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金
关键词
雷公藤
环氧二萜内酯
免疫抑制
Tripterygium wilfordii diterpne lactone expoxide lymphocyte proliferation immunosuppressive
