摘要
实验采用乙醇超声提取样品,HPLC梯度洗脱.菝葜皂苷的理论塔板数为5628,分离度为1.42,拖尾因子为1.01.回归方程为Y= 5×10^6X＋610 06,r=0.999 0.在0.4～2.0mg/mL之间的线性关系良好.日内和日间精密度的RSD均小于2.0%,重复性试验的RSD为0.6% （n= 5）,最低检测限为1.0μg/mL,加样回收率为96.5%、94.3%、97.2%,RSD（n=5）分别为2.7%、3.1%、2.6%.
The medicinal plant was extracted with ethanol and the extraction was repeated for three times.The theoretical plate number of astragaloside Ⅳ was 5628,the resolution 1.42 and the tailing factor 1.01.The calibration curve was linear in the range of 0.4～2.0mg/mL,Y= 5×10^6X ＋61006,R = 0.9990.The intra-day and inter-day precisions（RSD） were all less than 2.0%,the RSD of recurrence was 0.6%（n=5）.The limit of detection was 1.0 μg/ml,the recovery 96.5%,94.3%,97.2%,and RSD 2.7%,3.1%,2.6%（n=5） respectively for Sarsasapogenin.
出处
《贵州师范大学学报（自然科学版）》
CAS
2007年第3期98-101,共4页
Journal of Guizhou Normal University：Natural Sciences
关键词
HPLC-ELSD
菝葜皂苷
知母药材
high-performance liquid chromatography with evaporative light-scattering detection
Sarsasapogenin
Anemarrhenaasphodeloides Bge
