摘要
对贵州黄芪药材黄芪甲苷的含量进行了测定,采用甲醇超声提取样品,HPLC梯度洗脱.黄芪甲苷的理论塔板数为5588,分离度为1.42,拖尾因子为1.01.回归方程Y=1.4048X＋16.682,R=0.9990,在4.400-440.0μg/mL之间的线性关系良好.日内和日间精密度的RSD均小于1.5%,重复性试验的RSD为0.7%（n=5）,最低检测限为0.440μg/mL,加样回收率为98.9%、96.7%、99.8%,RSD（n=5）分别为2.8%、3.5%、2.5%.黄芪样品中黄芪甲苷的含量均符合《中华人民共和国药典》（2005版）标准.该方法缩减了黄芪药材的前处理步骤,节约了实验时间,适用于黄芪药材中黄芪甲苷含量测定;同时也证明黄芪的质量较好.
To develop a method for measurement of astragaloside Ⅳ in the Radix astragali from Guizhou province. The Radix astragali was extracted with methanol and the extraction repeated for three times. The theoretical plate number of astragaloside Ⅳ was 5 588, the resolution 1. 621 and the tailing factor 1. 113. The calibration curve was linear in the range of 4.400- 440.0μg/mL, Y = 1.404 8 X ＋ 16.682, R = 0.999 0 .The Intra-day and inter-day precision （RSD） were all less than 1.5%, the recurrence （RSD） was 0.67% （n = 5） . The limit of detection was 0. 440 μg/mL, the recovery 98.9%, 96.7 %, 99.8%, and RSD 2.8 % ,3.5 % ,2.5 % （ n = 5 ） respectively for astragaloside Ⅳ. Our developed method can shorten the time used for Radix astragali pretreatment, and is a simple and reliable method to determine the content of astragaloside Ⅳ in Radix astragali. The results also prove that the quality of Radix astragali in Guizhou Province is relatively good.
出处
《江西师范大学学报（自然科学版）》
CAS
北大核心
2007年第3期278-281,共4页
Journal of Jiangxi Normal University(Natural Science Edition)
关键词
HPLC-ELSD
黄芪
黄芪甲苷
黄芪药材
high-performance liquid chromatography with evaporative light-scattering detection
radix astragali
astragaloside Ⅳ
medicinal plants
