摘要
目的利用昆明小鼠体外受精模型观察巨噬细胞对小鼠卵受精率和囊胚形成率的影响。方法（1）预冷生理盐水注射小鼠腹腔,诱导巨噬细胞渗出,用贴壁法富集巨噬细胞。（2）取卵后随机把卵分成3组：对照组：小鼠卵单独培养,培养过程中无巨噬细胞共培养;实验组1：取卵后即与巨噬细胞共培养;实验组2：受精50~55 h后与巨噬细胞共培养。观察各组小鼠卵受精率和囊胚形成率。结果受精率实验组1（72.0%）明显低于对照组（91.0%）和实验组2（93.7%）,差异有统计学意义（P〈0.001）;受精卵的囊胚形成率实验组2（78.2%）明显高于对照组（60.3%）,差异有统计学意义（P〈0.001）;总囊胚形成率实验组2（73.2%）显著高于对照组和实验组1（分别为54.8%和50.0%）,具有显著的统计学差异（P〈0.001）。结论巨噬细胞降低小鼠卵子的受精率,增加囊胚形成率,在8细胞形成前后添加巨噬细胞共培养,能最大程度增加囊胚形成率。
Objective： To investigate the effect of co-culture with peritoneal macrophage on the fertilization rate and blastocyst formation rate of the mouse oocytes. Methods： A total of 15 Kunming mice aged 10-14 weeks underwent ovulation induction and oocyte collection. The oocytes were randomly divided into three groups. The oocytes in the first group （control group） were cultured without macrophages. The oocytes in the second group （experimental group 1） were co-cultured with macrophages right after egg collection, while the oocytes in the third group （experimental group 2） were cultured alone after egg retrieval, and co-cultured with macrophages from 8 cell stage. Results： The fertilization rates in control group （91.0%） and experimental group 2 （93.7%） were significantly higher than that in experimental group 1 （72.0%, P〈0. 001）. Blastocyst formation rate of the fertilized oocytes in the experimental group 2 （78.2%） was much higher than that of control group （60. 3%,P〈0. 001）. Blastocyst formation rate in experimental group 2 （73.2%） was much higher than that of control group （54.8%） and experimental group 1 （50. 0%）, （P〈0. 001 respectively）. Conclusions： Co-culture with peritoneal macrophages had an adverse influence on the fertilization process. Peritoneal macrophages could enhance the mouse embryonic development and increase the blastocyst formation rate.
出处
《生殖医学杂志》
CAS
2007年第4期269-273,共5页
Journal of Reproductive Medicine
关键词
巨噬细胞
共培养
小鼠卵
体外受精
囊胚形成
Macrophage
Co-culture
Mouse oocyte
In vitro fertilization
Blastocyst formation
