摘要
通过接种试验和再分离培养证明,极细枝孢是落叶松芽枯病的致病菌。在25℃下,病菌在PDA、麦芽糖、麦芽汁、基本培养基上都能生长,菌落初期白色,渐变淡绿色和暗绿色,7日后分生孢子梗伸长,与菌丝相似,直或弯曲,具分隔,长达800μm。分生孢子萌发的温度范围为5—30℃,最适温度为20℃,最适相对湿度为90%—100%,最低相对湿度为50%。菌丝生长和孢子产生最适pH值为pH4—6,葡萄糖和木糖为最佳碳源,天门冬素为最佳氮源,维生素H和维生素B_1对孢子产生作用很大。
Cladosporium tenuissimum Cook was proved to be a pathogen of the larch bud-blight by inoculation and retroculture. At temperature of 25℃, the fungi grows well on PDA, and media with malt and barley juice and element media and produces conidia. At first colony were white and gradually become light-green and dark green. After 7 days, conidiophore stretch, resembling the hyhae, straight or curved, septate, up to 800 μm long. The eonidia can germinate at 5—30℃ and the optimum temperature for conidia to germinate is 20℃. Germination usually occurs profusely higher humidity, 90% to 100% (R. H.), and is inhibited at about 50% (R. H.). The optimum pH for colony to grow and conidial production is 4—6. glucose and xylcose is the best source of carbon, and asparagine is the best source of nitrogen. Vitamin Hand vitamia B_1 have great effect on conidial production.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
1989年第3期38-41,共4页
Journal of Northeast Forestry University
关键词
枝孢
生物学特性
落叶松
芽枯病
Cladosporium
Biological characters
Larix
Bud blight