摘要
目的观察新生大鼠脑缺血时c-fos蛋白在海马区的表达特点,探讨c-fos蛋白表达与缺氧缺血性脑损伤(HIBD)的关系。方法48只7日龄新生SD大鼠随机分为对照组6只和实验组42只。给动物吸入含有920mL/L氮气和80mL/L氧气混合气体建立新生大鼠HIBD动物模型,分别在脑损伤后不同时间点(0.5、2、4、12、24、48、72h)断头处死动物,取海马组织,用免疫组织化学方法检测海马脑神经细胞凋亡及c-fos蛋白表达情况。结果海马区c-fos蛋白阳性细胞在HIBD后立即出现,2h达到高峰,持续至4h渐减低并持续至72h,各时间点阳性凋亡细胞的出现与c-fos蛋白呈负相关(r=-0.57P<0.05)。结论c-fos蛋白强表达于HIBD新生大鼠的海马缺血区和缺血周边区的神经元,与神经元的存活或死亡有一定联系。
Objective To explore the relationship of c - fos protein and cell apoptosis by observing the expression of c - fos protein in hippocampus of newborn rat with hypoxic - ischemic brain damage （HIBD）, Methods Forty - eight 7 - day SD neonatal rats were randomly divided into control group （n = 6） and experiment group （n = 42 ）. The models of HIBD were established in neonatal rats by inhaling the mixed gases of 920 mL/L N2 and 80 mL/L O2 , and the animals were sacrificed by dislocation their heads at different time points （0.5,1,3,6,12,24,48,72 h）, then the hippocampus were dissected by morphological analysis, Results The apoptotic cells appeared at the time point of 3 h, and reached the peak at 48 h,then decreased, The positive cell of c -fos protein increased from the time point of 30 min and reached the peak at 2 h and then decreased gradually, and there was a contrary tendency between the expression of c - fos protein and the number of damaged brain cells by HIBD（ r = - 0.57 P 〈 0.05 ）. Conclusion The expression of c - fos protein in hippocampus of rats with HIBD is stronger than other areas of brain,and there is something between cell apoptosis and c - fos protein.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第14期1097-1098,共2页
Journal of Applied Clinical Pediatrics
关键词
大鼠
新生
缺氧缺血
脑
C-FOS蛋白
凋亡
rat, newborn
hypoxic - ischemic brain
c - fos protein
apoptosis
