摘要
目的:探讨手术及化疗对卵巢癌患者外周血CD4+CD25+调节性T细胞(Treg)比例的影响。方法:选择39例卵巢癌患者,其中29例分别于手术前后采集外周血,另外10例于化疗前后采集。采用流式细胞法检测外周血CD4+CD25+细胞的比例;将分选的CD4+CD25+细胞和CD4+CD25-细胞按1∶1的比例混合培养后,利用BrdU法检测细胞增殖,并与单纯的CD4+CD25+细胞、CD4+CD25-细胞比较,以验证CD4+CD25+细胞的免疫抑制功能。结果:39例卵巢癌患者外周血CD4+CD25+细胞占淋巴细胞比例为5.92%±2.48%,占CD4+细胞的比例为15.32%±6.02%。CD4+CD25+与CD4+CD25-细胞混合培养组和单纯CD4+CD25-细胞组增殖细胞比例分别为32.15%±8.29%和56.38%±12.65%。手术前后CD4+CD25+细胞占CD4+细胞比例分别为14.74%±5.36%、11.86%±6.09%(P=0.015)。化疗前后CD4+CD25+细胞含量分别为5.70%±1.79%,7.46%±1.68%(P=0.031),化疗前后Tregs占CD4+细胞比例分别为14.11%±3.3%、19.56%±6.00%(P=0.039)。结论:卵巢癌患者手术后外周血CD4+CD25+细胞比例减少,提示其与肿瘤负荷相关;化疗后CD4+CD25+细胞增加,可能与化疗诱导的免疫抑制以及调节性T细胞的分化或增殖相关。
Objective： To observe the influence of surgery and chemotherapy on the percentage of CD4^＋CD25^＋ regulatory T cells （Treg） in peripheral blood （PB） of ovarian cancer patients, Methods： The prevalence of Treg in PB before surgery and after surgery was examined by flow cytometry method in 29 cases of patients suffering ovarian cancer. The influence of chemotherapy on the number of Treg was analyzed in 10 cases of patients. To determine regulatory properties, CD4^＋CD25^＋ （freshly isolated） and CD4^＋CD25^- T cells from tumor patients were cocuhured in a ratio of 1：1. Proliferation was measured after 4 days by BrdU assay. Results： The percentage of cells in peripheral blood of 39 cases was 5.92% ±2.48% in lymphocytes and 15.32%±6.02% in CD4^＋ T cells, respectively. The proportion of proliferating cells indicated by BrdU staining in the CD4^＋CD25^- cells co-cultured with CD4^＋CD25^＋ cells and purified CD4^＋CD25^- cells was 32.15%±8.29% and 56.38%±12.65% respectively, which testified the suppressive activity of CD4^＋CD25^＋ T cells. The prevalence of CD4^＋CD25^＋ cells in CD4^＋ cells decreased from 14.74%±5.36% to 11.86%±6.09% after surgery （P= 0.015）. However, the chemotherapy seemed to induce an expansion of this cell population. Conclusion： The percentage of Tregs in PB of ovarian cancer patients decreases after surgery, which indicats that the number of Treg may correlate with tumor burden. The chemotherapy may induce the differentiation or expansion of regulatory T cells.
出处
《天津医科大学学报》
2007年第2期217-220,共4页
Journal of Tianjin Medical University
