摘要
目的研究CD4+CD25+调节性T细胞在小鼠乳腺癌实验动物模型中的变化,并探讨其意义。方法以4T1接种BALB/c小鼠建立小鼠乳腺癌动物模型;分别以接种后1周和4周为荷瘤早、晚期;用流式细胞术(FACS)检测小鼠CD4+CD25+Foxp3+调节性T细胞在肿瘤局部及引流淋巴结中的比例变化;通过特异性增殖和杀伤实验观察早、晚期肿瘤局部的免疫应答。结果CD4+CD25+调节性T细胞在肿瘤浸润淋巴细胞中的比例晚期为7.36%±0.26%,高于早期4.47%±0.88%(P<0.05);在引流淋巴结中比例变化不大;肿瘤浸润淋巴细胞的抗原特异性增殖和杀伤能力明显减弱(P<0.05)。结论CD4+CD25+调节性T细胞在肿瘤局部存在富集,这可能是导致肿瘤局部免疫反应减弱的重要原因。
Purpose To analyze the change of CD4^＋CD25^＋ regulatory T cells in mammary carcinoma and to investigate their role in the tumor progress. Methods An experimental animal model of mouse mammary carcinoma was established by subcutaneous injection of 4T1 cells. The percentage and count of CD4^＋CD25^＋regulatory T cells in tumor infiltrating lymphocytes （TILs） and draining lymph node lymphocytes （DLNLs） derived from 4T1-bearing mice were detected in early and late stage of the tumor by FACS. Expression level of Foxp3 in CD4^＋CD25^＋ T cell was measured by FACS. The antigen specific lymphoproliferation and cytotoxicity of TILs was assayed. Results The percentages of CD4^＋CD25^＋regulatory T cells in the TILs were （7.36±0.26） % and （4.47±0.88） %, respectively in early and late stages. The count and percentage of CD4^＋CD25^＋ regulatory T cells in the TILs in the late stage of tumor were significantly higher than that in the early stage （P〈0.05）. The specific lymphoproliferation and cytotoxicity of TILs were significantly down-regulated in the late stage comparing to the early stage （P〈0.05）. Conclusions The enrichment of CD4^＋CD25^＋ regulatory T cells in tumor mass could be related to tumor progress.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2006年第6期736-740,共5页
Fudan University Journal of Medical Sciences
基金
上海市卫生局领军人才计划(LJ06011)资助
