摘要
目的建立广佛手药材HPLC指纹图谱,以便比较完整地反映广佛手的内在化学信息,全面评价广佛手药材质量,与其他产地佛手进行鉴别。方法应用HPLC法测定了12批佛手的指纹图谱,乙腈-0.5%冰醋酸为流动相进行梯度洗脱,检测波长290nm,体积流量1.0mL/min,柱温30℃,收集75min。结果建立了广佛手HPLC指纹图谱共有模式,并对不同产地药材进行了相似度比较。结论该方法稳定可靠,信息量大,为广佛手药材鉴别和质量评价提供了依据。
Objective To establish the HPLC fingerprints （HPLC-FPS） of Fructus Cirri Sarcodactylis （FCS） from Guangdong for reflecting the internal chemical information, evaluating its internal quality of FCS and identifying them from different cultural areas. Methods The HPLC-FPS of 12 FCS samples were obtained. The HPLC separation was performed on a Kromasil C18 analytical column by gradient eluting with acetonitrile-0. 5% acetic acid at the flow rate of 1.0 mL/min. The column temperature was 30℃, the UV detection wavelength was 290nm, and the collection time was 75 rain. Results The mutual mode of HPLC fingerprints was set up and the similar degrees to the crude drugs from different cultural areas were compared. Conclusion The method is stable, reliable, and with full information which can be used as a quality control item for FCS from Guangdong.
出处
《中草药》
CAS
CSCD
北大核心
2007年第7期1075-1077,共3页
Chinese Traditional and Herbal Drugs
基金
国家"十五"重大科技专项"创新药物和中药现代化"资助项目(2001BA701A38)
广东省科技厅重点项目(2004B33001010)
关键词
广佛手
指纹图谱
HPLC
Fructus Cirri Sarcodactylis （FCS） from Guangdong
fingerprint
HPLC
