摘要
目的:研究生地黄止血有效部位HPLC指纹图谱。方法:采用高效液相色谱法,Akasil-C18柱(4.6 mm×250 mm,5μm),乙腈-水梯度洗脱,检测波长210 nm,流速1.0 mL.min-1,柱温30℃。结果:确定了生地黄止血有效部位的10批指纹图谱共有模式,进行了相似度比较。结论:所建立的方法可用于生地黄止血有效部位指纹图谱测定,并可对其制剂的质量评价提供科学依据。
Objective：HPLC fingerprints of the hemostatic fraction of Rehmannia glutinosa were estab-lished.Method： Akasil C18（4.6 mm × 250 mm,5 μm） column was used and the acetonitrile-water was chosen as the mobile phase in a gradient mode.The column temperature was at 30 ℃ and the detection wavelength was at 210 nm.The detection time was 60 min,and the flow rate was 1.0 mL.min-1.Result：Fifteen characteristic peaks were indicated in HPLC fingerprints.The relative retention time is as of the common peaks were also determined.Conclu-sion： The method established is simple and accurate with a good reproducibility and will provide a reference stand-ard for the quality control of R.glutinosa.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第4期90-92,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
陕西省科技厅计划项目(2008K16-04
SJ08C239)
关键词
生地黄
止血
指纹图谱
Rehmannia glutinosa
hemostatic effect
fingerprints
