摘要
研究一氧化氮 （ＮＯ）和内皮素 （ＥＴ）两者在脑缺血中的关系，建立免大脑中动脉阻断（ＭＣＡＯ）模型，分别测定脑组织ＮＯ、ＥＴ－１含量及一氧化氮合成酶（ＮＯＳ）活性：应用ＮＯＳ抑制剂、ＥＴ受体拮抗剂分别观察它们对ＥＴ－１、 ＮＯ含量的影响。结果：脑缺血早期 （２ ｈ）ＮＯ含量、 ＮＯＳ活性均降低，脑缺血后 ４～４８ ｈ ＮＯ含量， ＮＯＳ活性显著增加；应用 ＮＯＳ抑制剂后 ＥＴ—ｌ含量增加， ＥＴＢ受体拮抗剂后 ＮＯ含量降低。结论： ＮＯ可能抑制 ＥＴ—ｌ产生，反过来ＥＴ—１可能促进ＮＯ形成．其机理尚待进一步研究。
To study the relationship between Nitric Oxide and Endothelin-l in cerebral ischemia. METHODS The contents of Nitric Oxide (NO), Endothelin-l (ET-l) and the activity of nitric oxide synthase (NOS) were determined after in rabbit of cerebral ischemia from middle cerebral artery occlusion model. We also studied the effect of antagonist of ETB receptor and the inhibitor of NOS on the contents of NO and ET-1, respectively. RESULTS The NO content, NOS activity decreased in the ischemic early stage (2h), and increased in middle and later stage (4- 48 h). The antagonist of ETB receptor decreased NO contents and L-NNA enhanced the co 'tents of ET-l. CONCLUSIONS NO might inhibit ET-l production, however, ET-l might promote NO production.
出处
《上海医科大学学报》
CSCD
1997年第1期45-47,共3页
Journal of Fudan University(Medical Science)
