摘要
目的：建立葶苈降血脂片中熊果酸的含量的测定方法。方法：采用高效液相色谱法,色谱柱为ZORB-AXSB-C18色谱柱（4.6mm×150mm,5μm）,检测波长为215nm,流动相为甲醇-水-醋酸铵-乙腈（9∶25∶0.8∶65）,柱温为室温,流速为1.0mL·min^-1。结果：熊果酸的含量在72-360μg范围内与峰面积呈良好的线性关系,r=0.9995,平均回收率为100.3%（n=5）。结论：方法操作简便,快速,结果准确可靠。
Objective ： To establish a method for determination of ursolic acid in Tingli jiangxuezhi tablets. Methods： To determine ursolic acid in Tingli jiangxuezhi tablets by HPLC. The liquid chromatography was carried out by a ZORBAX SB - C 18 （4. 6 mm× 150 mm, 5μm） with dectection wavelenth at 215nm. The mobile phase was methanol - water - acetic - acetonitrile（9： 25： 0.8： 65）. The flow rate was 1.0mL · min^-1. Results： The carl/bration curve was linear in the range of 72 -360μg for ursolic acid（ r =0. 9995）. The average recovery（ n =5）was 100, 3%. Concision： The method is rapid, accurate and reliable.
出处
《中华中医药学刊》
CAS
2007年第7期1504-1505,共2页
Chinese Archives of Traditional Chinese Medicine
