摘要
为了分析石棉相关肿瘤ｐ５３基因的突变特点，对石蜡包埋的石棉相关肿瘤ｐ５３基因突变体蛋白的表达进行了免疫组化观察，提取染色体ＤＮＡ，对ｐ５３基因的第５、７、８外显子进行ＰＣＲ－ＳＳＣＰ及测序分析。免疫组化观察发现：在分析的１０例病例中有５例阳性。ＰＣＲ－ＳＳＣＰ分析发现７例（８处）发生突变，其中４处集中在第８外显子上。５例腺癌中有４例发生ｐ５３基因突变。测序发现热点区突变。提示：石棉相关肿瘤ｐ５３基因突变率高。
p53 gene mutations were detected in asbestos related cancer using immunohistochemical and PCR SSCP methods.Ten cases of asbestos related cancer were collected.The cancer tissues were embedded in paraffin and stored for 14.6 years on average.Immunohistochemical study revealed five p53 mutation positive cases.Chromosome DNA was extracted from paraffin embedded specimens.p53 mutations(8 mutation sites) were found on exons 5,7 and 8 in seven cases by PCR SSCP analysis.Of 8 mutations,4 were clustered in exon 8.A high mutation frequency was noticed in adenocarcinoma(80%).Point mutations were noticed in “hotspots” of p53 mutation.The results suggest that the mutational spectrum of asbestos related cancer might be different from that of non asbestos related cancer.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
1997年第3期150-153,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
中国预防医学科学院科研基金
中国自然科学基金委科研基金
