摘要
用两步PCR法，成功地从石蜡包埋组织DNA中扩增了P16INK4exon2片段；并用单链构象多态（SSCP）分析PCR产物的差异．在32例肝癌原发灶中，发现P16INK4exon2缺失突变6例、SSCP可检出突变1例；在48例肝癌转移灶中，发现P16INK4exon2缺失突变11例、SSCP可检出突变2例．
The P16INK4exon2 has been successfully amplifed by two-step-PCR from tissues embeded in parraffin, and the differences of the PCR products were analyzed by SSCP. Six deletions detected by PCR and one SSCP-detectable mutation were found in 32 idiopathy liver cancers while eleven deletions detected by PCR and two SSCP-detectable mutations in 48 liver cancers.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第4期537-541,共5页
Journal of Fudan University：Natural Science
基金
国家自然科学基金
