摘要
目的:观察新型多孔型磷酸钙骨水泥骨内移植的生物学特点,为其临床运用提供实验依据。方法:实验于2006-09/2007-02在南方医科大学珠江医院神经外科实验室完成。①选用健康成熟新西兰大白兔20只,在双侧股骨外侧髁部建立骨缺损模式,钻取直径5mm,孔深6mm的柱状圆洞。其中左侧为多孔型磷酸钙骨水泥组,右侧为对照组。②多孔型磷酸钙骨水泥组将多孔型磷酸钙骨水泥(液粉比为0.4)用专用注射器注入骨孔内并使磷酸钙骨水泥外溢,同时指压2min至骨水泥凝固;对照组造成骨缺损后充分止血缝合伤口。③于术后4,8,12和16周分别处死新西兰兔(n=5),通过光镜及电镜观察多孔型磷酸钙骨水泥在骨组织内的生物相容性和成骨方式。结果:①所有实验动物无一死亡,仅有1只兔术后术区骨折(对照侧)。②光镜观察结果:在多孔型磷酸钙骨水泥组,术后4周时可见材料外缘出现活细胞网状结构;8周时材料边缘可见钉突状或伪足状新生骨小梁伸入其内,同时其表面可见部分软骨内化骨;12周时,边界可见新生骨小梁明显增多、增粗、增长,向材料内部生长,骨小梁边缘可见足量破骨和成骨细胞;16周时清晰可见材料部分降解后形成的空隙,沿孔隙或空隙伸入材料的新生骨小梁与材料交织在一起,骨小梁边缘和部分材料孔隙内可见大量成骨细胞和破骨细胞分布。而对照组术后早期在骨洞内由机化后的血肿组织填充,炎症反应明显,8～12周时纤维瘢痕形成,16周时未见有新骨形成。③电镜观察结果:在低倍扫描电镜下发现,新型多孔型磷酸钙骨水泥的孔径在200～400μm之间分布,大部分孔隙与孔隙之间有90μm左右的小孔道贯通,类似松质骨结构。在骨内植入8周时高倍扫描电镜下观察,在骨-骨水泥交接面可见新生骨小梁沿着磷酸钙骨水泥的孔隙伸长入其内部,两者之间紧密结合。16周时,磷酸钙骨水泥密
AIM： To evaluate the biological characters of a neo-type porous calcium phosphate cement （CPC） as a bone graft material, to provide basic data for its clinical application. METHODS： The experiment was conducted at the laboratory of Department of Neurosurgery, Zhujiang Hospital of Southern Medical University from September 2006 to February 2007. （1）Twenty healthy adult New Zealand white rabbits were selected, and the models of bone defects were established at the bilateral femoral condyles by drilling a 5 mm in diameter and 6 mm in depth pillar hole. （2）The left side served as CPC group, and filled with neo-type pore CPC （the ratio of solution and power was 0.4） by the special injector until the cement overflowed the pore, and pressed it for 2 minutes till the cement concreted; the right side acted as control, which was sutured after modeling. （3）Biocompatibility and bone formation style of CPC were observed under light microscope and scanning electron microscope after five animals were sacrificed respectively at weeks 4, 8, 12 and 16 after surgery. RESULTS：（1）No rabbits died besides one with bone fracture in the control side. （2）By the light microscope observation, the net structure of active cells in the CPC group was observed surround the material and there were prosperous cell growth inside the material 4 weeks after the operation; 8 weeks after the operation, the new formation of pseudopodia bone trabecular extended into the material and the endochondrale ossification was also found on the surface of the material; the newly formed trabecular became thick and swell and extended into the material, and a large amount of osteoclasts and osteoblasts were observed 12 weeks after the operation; the cellule formed by partially degradation of material was clear and the newly formed bone trabecula extended into the cellule, moreover, plenty of osteoclasts and osteoblasts distributed into the bone trabecula verge and cellule. In the control group, the bone defect was filled with the hem
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第18期3496-3499,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金(50172015)
广州市科技项目(200523-D2041)
南方医科大学珠江医院院长基金(2006)~~
