摘要
目的通过研究COX-2和VEGF在胃腺癌中的表达,探讨其在胃腺癌淋巴结转移中的作用。方法采用免疫组织化学SP法对58例伴有淋巴结转移(N+)的胃腺癌患者和33例无淋巴结转移(N-)的胃腺癌患者COX-2和VEGF表达进行检测。结果淋巴结转移组(Ⅰ-Ⅱ期)中COX-2的表达阳性率为75%,无淋巴结转移标本中COX-2的表达阳性率为54.5%,两组之间差异无统计学意义(P>0.05);胃癌Ⅲ-Ⅳ期COX-2表达阳性率(86%)明显高于Ⅰ-Ⅱ期COX-2表达阳性率(58.5%),两组差异显著(P<0.05);COX-2阳性表达组发生淋巴结转移高于COX-2阴性表达组(P<0.05);在淋巴结转移组的标本中COX-2和VEGF的表达有相关性(P<0.05)。结论淋巴结转移组中的COX-2表达和同期无淋巴结转移组中的COX-2表达无明显差异,提示COX-2在早期胃腺癌淋巴结转移中的作用仍有待进一步探讨。COX-2的高表达与肿瘤转移有相关性,其作用可能通过调控VEGF途径来实现。
Objective To explone the relationship between lymph node metastasis and expression level of COX-2 and VEGF in gastric adenocarcinoma. Methods COX-2 and VEGF were detected in gastric adenocarcinoma with lymph node metastasis （58 cases） and without lymph node metastasis （33 cases） by using immunohistochemical SP method. Results The postive expression rate of COX-2 in the cases of clinical stage Ⅰ to Ⅱwith lymph node metastasis （75 % ） was not significantly higher than those without lymph node metastasis cases（P 〉 0.05 ）. The positive expression rate of COX-2 in the cases of clinical stage from Ⅲ to IV was significantly higher than that of the stage Ⅰto Ⅱ. The cases with COX-2 positive expression have more lymph node metastasis than those without COX-2 expression （P 〈 0.05）. There was a positive correlationship between COX-2 and VEGF in gastric adenocarcinoma with lymph node metastasis. Conclusion The expression of COX-2 in gastric adenocarcinoma of clinical stage Ⅰ and Ⅱwith lymph node metastasis is not different with those without lymph node metastasis. Overexpression of COX-2 played a critical role in the development of gastric carcinoma and lymph node metastasis by upregulating VEGF.
出处
《胃肠病学和肝病学杂志》
CAS
2007年第2期136-138,共3页
Chinese Journal of Gastroenterology and Hepatology
关键词
COX-2
胃腺癌
淋巴结转移
免疫组织化学
Cyclooxygenase-2
Gastric carcinoma
Lymph node Metastases
Immunohistochemistry
