摘要
背景:内脏感觉过敏是功能性胃肠病的重要病理生理机制之一。快动眼(REM)睡眠剥夺可降低大鼠内脏感觉,但具体机制不清。目的:研究内源性阿片肽系统是否参与构成REM睡眠剥夺所致大鼠内脏感觉降低的机制。方法:24只雄性Sprague-Dawley大鼠随机分为实验对照组、睡眠剥夺组和睡眠剥夺加阿片受体拮抗剂纳洛酮干预组(纳络酮组)。采用花瓶技术制作REM睡眠剥夺大鼠模型。睡眠剥夺持续48h后行结直肠扩张,记录腹壁肌电图,观察疼痛感觉阈值,以反映内脏感觉功能的变化。采用逆转录聚合酶链反应(RT-PCR)测定大鼠结直肠和丘脑阿片受体μ、κmRNA表达的变化。结果:给予不同压力扩张刺激后,睡眠剥夺组腹外斜肌放电次数显著低于实验对照组,疼痛感觉阈值高于实验对照组(P<0.05);纳洛酮组放电次数显著高于睡眠剥夺组,疼痛感觉阈值低于睡眠剥夺组(P<0.05),与实验对照组相比则均无显著差异。与实验对照组相比,睡眠剥夺组结直肠阿片受体κmRNA表达上调(P<0.05),丘脑κ受体mRNA表达无明显变化,上述部位μ受体mRNA表达均无明显变化。结论:REM睡眠剥夺降低大鼠内脏感觉的作用与内源性阿片肽和阿片受体κ表达上调有关。
Background： Visceral hypersensitivity is one of the important pathophysiologic mechanisms of functional gastrointestinal diseases. Rapid eye movement （REM） sleep deprivation can decrease the visceral sensation of rats, however, its mechanism has not been known clearly. Aims： To investigate whether the endogenous opioid peptides system is involved in the mechanisms of REM sleep deprivation-induced rats＇ visceral hyposensifivity. Methods： Twenty-four male Sprague-Dawley rats were randomly divided into three groups： cage-yoked control （YC group）, REM sleep deprivation （SD group） and naloxone （an opioid receptor antagonist） intervention after REM sleep deprivation （naloxone group）. Flower pot technique was used to construct REM sleep deprivation rat model. Colorectal distension （CRD） was performed 48 hours after the beginning of sleep deprivation. Both abdominal electromyogram and threshold of pain perception in response to CRD were used to assess the visceral sensitivity. Expression of opioid receptors μ and K mRNA in colorectum and thalamus was detected by reverse transcriptase polymerase chain reaction （RT-PCR）. Results： After sleep deprivation, the abdominal electrical activity frequency of external oblique muscle responding to CRD in the SD group was significantly lower than that in the YC group while the pain threshold was higher than that in the YC group （P〈0.05）. In naloxone group, the abdominal electrical activity frequency increased and the pain threshold decreased as compared with those in the SD group （P〈0.05）. But no significant differences of the two indices were observed between naloxone group and YC group. The colorectal opioid receptor K mRNA expression was upregulated in the SD group when compared with that in the YC group （P〈0.05）, while the expression of μ receptor and thalamic κ receptor mRNA were not affected by sleep deprivation. Conclusions： Endogenous opioid peptides and upregulation of opioid receptor κ may play active roles in
出处
《胃肠病学》
2007年第4期218-223,共6页
Chinese Journal of Gastroenterology
关键词
睡眠剥夺
内脏感觉
结直肠扩张
阿片样肽类
受体
阿片样
纳络酮
Sleep Deprivation
Visceral Sensitivity
Colorectal Distention
Opioid Peptides
Receptors, Opioid
Naloxone
