摘要
目的:研究乳腺癌组织中信号转导和转录激活因子3(STAT3)和细胞因子信号转导抑制分子3(SOCS3)的表达与肿瘤分化、浸润、转移的关系。方法:应用组织芯片和免疫组织化学Envision法检测71例乳腺癌组织和41例非癌组织中STAT3、磷酸化STAT3、SOCS3的表达情况及其与临床病理参数的关系。结果:(1)在71例乳腺癌中STAT3、磷酸化STAT3和SOCS3的阳性表达率分别为78.9%、69.0%和29.6%,与对照组比较,差异有统计学意义(P<0.01,P<0.01,P<0.05);(2)STAT3、磷酸化STAT3表达与乳腺癌的组织学分级、腋窝淋巴结转移、临床分期呈正相关(P<0.05,P<0.01),与患者年龄、瘤体大小和组织学类型无关(P>0.05);SOCS3表达与乳腺癌组织学分级、腋窝淋巴结转移呈负相关(P<0.05),与患者年龄、组织学类型、瘤体大小和临床分期无关(P>0.05);(3)乳腺癌组织中STAT3、磷酸化STAT3表达与SOCS3表达分别呈负相关(P<0.01)。结论:乳腺癌STAT3、磷酸化STAT3的高表达和SOCS3的缺失表达与肿瘤发生发展、浸润转移密切相关;对其检测有助于判断乳腺癌的恶性程度及生物学行为。
Objective： To investigate the expressions of signal transducer and activators of transcription 3 （ STAT3 ） and suppressor of cytokine signaling 3 （SOCS3） protein in the breast carcinoma tissue and their relationship with tumor differentiation, invasion, and metastasis. Methods： The expression of STAT3 ,phospho-STAT3 and SOCS3 protein were determined by tissue microarray and immunohistochemistry with EnVision system in 71 cases of archival breast carcinoma tissues and 41 cases of tissues. The relationship between their expression and the clinical pathological parameters were analyzed. Results： （ 1 ） The positive rates of STAT3, phospho-STAT3, and SOCS3 was 78.9%, 69.0%, and 29.6%, respectively. The difference was significantly different compared with control （P 〈0.01, P 〈0.01, and P 〈0.05）. （2） The expressions of STAT3 and phospho-STAT3 were positively related with the histological grade, the axillary lymph node metastasis, and the clinical stage （P 〈0.01 ）, but not related with the age, the tumor size, and the histological grade of breast carcinoma tissues （P 〉 0.05 ）. The expression of SOCS3 was negatively related with the histopathologic grade and the axillary lymph node metastasis （P 〈 0.05）, but was not related with the age, histological type, the tumor size, and the clinical stage （ P 〉 0.05 ）. （ 3 ） The expression of STAT3 and phospho-STAT3 had negative correlation with the expression of SOCS3 in breast carcinoma tissues （P 〈 0.01 ）. Conclusion： The overexpressions of STAT3 and phospho-STAT3 and the down-regulated expression of SOCS3 closcly correlated with the tumor carcinogenesis, progression, invasion, and metastasis of breast carcinoma. Detection of their expression is helpful in accessing the malignant degree and the biological behaviors of breast carcinoma.
出处
《肿瘤》
CAS
CSCD
北大核心
2007年第4期298-302,共5页
Tumor
关键词
乳腺肿瘤
转录激活因子3
细胞因子信号转导蛋白抑制因子
免疫组织化学
组织芯片
Breast neoplasms
Activating transcription factor 3
Suppressor of cytokine signaling proteins
Immunohisto-chemistry
Tissue microarray
