摘要
目的探讨补体C9、核因子κB65(NF-κB65)在大鼠实验性脑出血后血肿周围的表达及黄芪多糖干预后对其表达的影响。方法雄性SD大鼠69只,随机分为假手术组、模型组及治疗组,每组23只。根据动物处死时间又分为6h和1、3、5d4个亚组,每组每个时间点取5只大鼠用于免疫组化染色,3d时间点取3只用于透射电镜检查。模型组与治疗组采用Ⅶ型胶原酶诱导脑出血模型;假手术组以等量等渗盐水代替Ⅶ型胶原酶。治疗组于术后2h给予黄芪多糖2ml(25mg/kg),腹腔注射,1次/d。分别于6h和1、3、5d对大鼠神经行为学评分,采用免疫组化方法检测不同时间点血肿周围组织C9和NF-κB65表达的变化。透射电镜观察血肿周围神经细胞超微结构的变化。结果模型组与治疗组均于脑出血后6h开始表达补体C9,24h达高峰;6h开始表达NF-κB65,3d达高峰。各时间点表达均高于假手术组(P<0.050.01);NF-κB65的表达与C9的表达呈正相关关系(r=0.752,P<0.05)。与模型组比较,治疗组3、5d大鼠神经行为学评分明显减小(P<0.05),神经细胞超微结构改变亦比模型组减轻。结论补体C9和NF-κB65参与了脑出血后神经细胞的损伤;经黄芪多糖干预后,可能通过抑制NF-κB65及补体C9表达发挥神经保护作用。
Objective To discuss the expressions of complement （C9） and nuclear factor-kappa B （ NF-κB65 ） in perihematomal tissue following experimental intracerebral hemorrhage （ICH） in rats and the effect of astragalus polysaccharides （APS） intervention on them. Methods Sixty-nine male SD rats were randomly allocated into sham-operation, model, and treatment groups （n =23 in each group）, They were redivided into 6 hour, 1, 3, and 5 day subgroups according to the time of sacrifice of animals. Five rats in each subgroup were selected for immunohistochemical staining at each time point. Three rats were select for transmission electron microscope examination at the 3 day time point. ICH model was induced by collagenase Ⅶ in the model group and treatment group. The collagenase Ⅶ were replaced with an equal volume of isotonic saline solution in the sham-operation group. Two hours after procedures, APS 2 mL （25 mg/kg） was administered intraperitoneally once a day in the treatment group. The neurological behavioral scores in rats were evaluated at 6 hour, 1, 3, and 5 day intervals. The changes of C9 and NF-κB65 expressions in perihematomal tissue at different time intervals were detected with immunohistochemical method. The ultrastructure changes of perihematomal neurons were observed with transmission electron microscope 3 days after the procedures, Results C9 began to express 6 hours after 1CH and reached a peak at 2d hours in the model group and treatment group; NF-κB65 began to express at 6 hours and reached a peak at 3 days. The expressions at all time intervals in both groups were higher than those in the control group （P 〈0. 05 -0. 01 ）. The expression of NF-κB65 was positively correlated that of C9 （r = 0. 752, P 〈 0. 05 ）. The neurological behavior scores of the rats in the treatment group decreased significantly at 3 and 5 days （P 〈0. 05） as compared with the model group, and the changed neuronal ultrastructure was also milder than the model group. Conclusion C9 and NF-�
出处
《中国脑血管病杂志》
CAS
2007年第1期26-31,共6页
Chinese Journal of Cerebrovascular Diseases
关键词
脑出血
补体C9
核因子ΚB
黄芪多糖
Cerebral hemorrhage
Complement 9
NF-kappa B
Astragalus polysaccharides
