摘要
目的探讨甲磺酸卡莫他特(camostat mesilate,CM)对大鼠脑出血(intracerebral hemorrhage,ICH)后脑水肿形成的相关免疫学机制。方法将90只SD雄性大鼠随机分成假手术组、ICH对照组及CM治疗组;干湿法观察血肿周围脑组织含水量;免疫组化法分析血肿周围脑组织补体片段C9表达情况。结果 ICH对照组和CM治疗组的脑组织含水量显著高于假手术组差异有统计学意义(P<0.05);CM治疗组的脑组织含水量显著低于ICH对照组差异有统计学意义(P<0.05)。假手术组在血肿周围脑组织中未见补体C9表达;CM治疗组的补体C9表达阳性细胞数显著低于ICH对照组差异有统计学意义(P<0.05)。脑组织含水量与补体C9表达阳性细胞数均呈正相关。结论 CM对大鼠ICH具有保护作用,其作用机制是通过阻碍补体级联反应来发挥对ICH后脑水肿形成的治疗作用。
Objective To explore the immune mechanism of Camostat Mesilate on the formation of brain edema after ICH. Methods To divide SD rats into groups：90 SD rats were randomly divided into three groups （30 per group）, named sham operation group, ICH control group and Camostat Mesilate treating group. Water contents of brain tissue circum-hematoma would be detected by dry-wet weigh method at each observation time point. Use immunohistochemistry to detect Complement C9 fragment of brain tissue circum-hematoma at each observation time point. Results Water contents of brain tissue circum-hematoma in ICH control group and Camostat Mesilate treating group were significantly higher than those in sham operation group （P〈0. 05）. Water contents of brain tissue circum-hematoma in Camostat Mesilate treating group were significantly lower than those in ICH control group （P〈0. 05）. There is no complement C9 expression in sham operation group. The number of positive cells of complement C9 in Camostat Mesilate treating group were significantly lower than those in ICH control group （ P〈0. 05 ） . Water contents of brain tissue circum-hematoma was siginificantly positively correlated with the number of positive cells of complement C9. Conclusion After intracerebral hemorrhage, the application of Camostat Mesilate special to protect neurological function. Camostat Mesilate ex-erted treatment effect on the formation of brain edema by blocking complement cascade.
出处
《四川医学》
CAS
2015年第4期488-491,共4页
Sichuan Medical Journal
