摘要
采用正交实验设计,利用超声法从琐琐葡萄中提取生物活性成分总三萜,比色法测定总三萜的含量。结果正交实验设计的四个因素:甲醇浓度、样品粒度、超声功率和提取时间对总三萜提取率均有影响(P<0.05)。超声法提取琐琐葡萄中总三萜的最佳提取工艺为:样品粉碎后过80目筛,纯甲醇为溶媒,在功率为210W时超声提取10min,总三萜提取率最高。
Extract and quantitative analysis of biological active ingredients total triterpenes in Suosuo grape.Using orthogonal experimental design, extracted with ultrasonic and detected by colorimetric. In orthogonal experimental design four selected factors： concentration of methanol, granularity of samples, power of ultrasonic and time of extract are all factors which can effect the extract rate of total triterpenes （p〈0.05）. Under 80-mesh, pure methanol for solvents, 210 Wpower for 10 minutes, using ultrasonic method, total triterpenes in Suosuo Grape can be highly extracted.
出处
《食品研究与开发》
CAS
北大核心
2007年第4期8-11,共4页
Food Research and Development
基金
国家自然科学基金资助项目
项目编号:30660157
关键词
琐琐葡萄
总三萜
超声
比色法
Suosuo grape
total triterpene
ultrasonic
colorimetric method
