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高效液相色谱法测定葡萄皮和葡萄籽中白藜芦醇的含量 被引量:27

Quantitative analysis of resveratrol from grape seeds and grape skins by high performance liquid chromatography method
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摘要 测定不同品种葡萄皮和葡萄籽及酿酒后的葡萄皮渣中白藜芦醇的含量。应用乙腈 -水 (2 6∶74 )为流动相 ,检测波长 (λ)为 30 3nm ,柱温为 35℃ ,色谱柱 :SUPELCOSILTMLC 18(2 5 0mm× 4 6mm ,5 μm)。结果显示 ,葡萄皮中的白藜芦醇含量高于葡萄籽 ;不同品种葡萄皮中白藜芦醇含量差异较大 ,琐琐葡萄最低为0 985 0 μg g ,红克里米斯克葡萄最高为 15 4 195 μg g;同一产地同一品种葡萄皮中白藜芦醇含量依次为酿酒后的葡萄皮 >新鲜葡萄皮 >陈旧葡萄皮 。 In order to perform a quantitative analysis of resveratrol from grape seeds, grape skins and waste residue of grape winery, acetonitrile-water(26:74) was applied as the fluxion phase. The detective wave-length(λ) was 303nm. Colume temperature was 35℃.The type of chrimato pattern columewas SupelcosilLTMLC-18(250mm×4.6mm,5μm).Results showed that the contents of resveratrol from grape skins were higher than those of grape seeds. Among different kinds of grape skins, resveratrol contents varied. The resveratrol content of Suo Suo grape skin was the lowest (0.9850μg/g). Among the same kinds of grape skins from the same production area, the resveratrol content was highest in the skin of waste residue of grape winery followed by the fresh grape skin and the old grape skin ranked thirdly, and the differences of resveratrol contents were statistically significant.
出处 《卫生研究》 CAS CSCD 北大核心 2003年第5期490-492,共3页 Journal of Hygiene Research
关键词 葡萄皮 葡萄籽 白藜芦醇 高效液相色谱法 含量测定 grape skin, grape seed, resveratol, high performance liquid chromatography method
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