摘要
目的用原子吸收光谱法测定琐琐葡萄中12种无机元素的含量。方法称取5份经粉碎、干燥恒重后的琐琐葡萄样品1.000 0 g置于150 ml三角烧瓶中,加入硝酸+高氯酸(4+1)混合溶液20 ml,电热板消化后,用超纯水稀释至刻度,在仪器工作条件下用3%稀硝酸做空白测定吸光度。用火焰原子吸收光谱法测定了琐琐葡萄中K、Ca、Na、Mg、Fe、Mn、Zn、Cu、Cr元素的含量,Cd、Pb、Se元素含量用石墨炉原子吸收光谱法测定。结果原子吸收光谱法测定的线性相关系数为0.997 9～0.999 9,相对标准偏差在0.19%～4.55%之间。样品中的K、Ca、Na、Fe、Mn、Cu、Cd、Cr、Se含量分别为11 906.50、1 409.01、479.03、208.80、8.97、5.21、0.06、0.25、1.98μg/g,Mg、Zn和Pb未检出。结论原子吸收光谱法检测琐琐葡萄中的无机元素具有良好的准确度和精密度;琐琐葡萄中K、Ca、Na、Fe、Se含量较高;琐琐葡萄抗炎作用可能与其中无机元素含量较高有关。
Objective To determine 12 inorganic elements in Vitis amurensis by atomic absorption spectrometry（AAS）.Methods Samples were dried and crushed with a mechanical device.Weighing 1.000 0 g of crushed Vitis amurensis and putting into a 150 ml Erlenmeyer flask.Adding 20 ml of acid digestion reagent（HNO3 ∶HClO4=4∶1 by volume）and then heated on an electric hot plate.The contents of K,Ca,Na,Mg,Fe,Mn,Zn,Cu and Cr in Vitis amurensis were determined by flame atomic absorption spectrometry,the contents of Cd,Pb and Se were determined by graphite furnace atomic absorption spectrometry.Five parallel samples and blanks were required for each Vitis amurensis sample.Results The correlation coefficient of standard curve determined by atomic absorption spectrometry was 0.997 9-0.999 9,RSD was 0.19%-4.55%.The contents of K,Ca,Na,Fe,Mn,Cu,Cd,Cr and Se in Vitis amurensis were 11 906.50,1 409.01,479.03,208.80,8.97,5.21,0.06,0.25 and 1.98 μg/g,and the contents of Mg,Zn and Pb were not be detected.Conclusion The determination of inorganic elements in Vitis amurensis with AAS was good at accuracy and precision;the contents of K,Ca,Na,Fe and Se were higher;The anti-inflammatory effect of Vitis amurensis might be relevant to its higher inorganic element contents.
出处
《中国食品卫生杂志》
北大核心
2011年第5期411-413,共3页
Chinese Journal of Food Hygiene
基金
国家自然科学基金项目(30660157)
教育部博士点基金项目(200807600002)
关键词
琐琐葡萄
无机元素
原子吸收光谱法
食品
Vitis amurensis
inorganic elements
atomic absorption spectrometry
food
