摘要
目的建立用高效液相色谱法同时测定中药材紫草中左旋紫草素和β,β-二甲基丙烯酰阿卡宁含量。方法色谱柱为Hypersil BDS C18柱(250mm×4．6mm),室温,流动相为乙腈-水-甲酸为(680:320:5),流速为1．0mL·min-1,检测波长为516nm。结果左旋紫草素在0．0711～14．255μg的范围内线性关系良好(r=0．9999),β,β-二甲基丙烯酰阿卡宁在8．71～174．5βg范围内线性关系良好(r=0.9999),两个成分平均回收率均在95～105％之间。结论该方法快速、简便,分离度好,能同时测定中药材紫草中左旋紫草素和β,β－二甲基内炳酰阿卡宁含量,可用于紫草的质量控制。
OBJECTIVE To develop a RP-HPLC method for determination of Shikonin and β,β- dimethylacrylalkannin in Radix arnebiae. METHODS Shikonin and β,β-dimethylacrylalkannin were separated on Hypersil C18 column （250mm × 4.6mm, 5μm）and detected at 516nm by using Acetonitrile-H2O-HCO2H （680 ： 320 ： 5） as the mobile phase, at flow rate of 1.0 mL · min^-1 .RESULTS Shikonin and β, β-dimethylacrylalkannin had good linearity in the range of 0. 0711-14. 255μg（r=0. 9999） and 8.71-174.5μg（r =0. 9999）respectively. The average recoveries were between 95-105%. CONCLUSION This method is rapid, simple and accurate for quantitative analysis of Radix arnebiae.
出处
《海峡药学》
2007年第4期35-38,共4页
Strait Pharmaceutical Journal
