摘要
目的建立紫草油中左旋紫草素的含量测定方法。方法采用HPLC法测定紫草油中左旋紫草素的含量,色谱柱:岛津Shim-packVP-ODS柱(4.6mm×250mm);甲醇-0.025mol/L磷酸(85∶15)为流动相;检测波长:516nm;柱温:25℃;进样量:20μL。结果左旋紫草素在11.2μg/mL～33.6μg/mL浓度范围内线性关系良好(r=0.9998);平均回收率为101.03%,RSD=1.90%(n=5)。结论该方法简便、准确,能排除其他成分的干扰,可用于紫草油的质量控制和评价。
Objective To establish a method for the content determination of shikonin in Radix Arnebiae Oil by HPLC.Methods The content of shikonin was determined by HPLC and the chromatographic conditions were: Shim-pack VP-ODS column(4.6 mm×250 mm),the mobile phase consisting of methanol-0.025 mol/L H3PO4(85 ∶15),detection wavelength being 516 nm,tempeature at 25 ℃and the flow rate being 20 μL.Results A good linearity of shikonin was in the range of 11.2 μg/mL～33.6 μg/mL(r=0.9998),and the average recovery rate was 101.03 %,RSD=1.91%.Conclusion This method was simple,accurate and with a good reproducibility and can be used for the quality control of Radix Amebiae Oil.
出处
《中药新药与临床药理》
CAS
CSCD
2005年第1期66-68,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
