摘要
目的：优选紫草素明胶-海藻酸钠微囊的制备工艺条件。方法：以紫草素微囊载药量、包封率、粒径大小及分布为制备工艺的优化指标,通过单因素试验和L9（34）正交试验,考察了乳化剂种类、乳化温度、明胶/海藻酸钠最佳浓度与比例、芯壁比、Ca Cl2浓度等因素对微囊形成的影响。结果：紫草素复合微囊的最佳制备条件为：2%的明胶和海藻酸钠（比例为1∶3）,芯壁比为1∶1,Ca Cl2浓度为5%,吐温-司盘（1∶1）为乳化剂,乳化温度55℃;所得微囊成球性好、粒径分布均匀,载药量和包封率分别为18.27%、81.90%。结论：优选工艺所制备的紫草素明胶-海藻酸钠微囊包封率和载药率都较高,粒径均一,为紫草素微囊的进一步研究奠定基础。
Objective：To optimize the preparation condition for microencapsulation of shikonin. Methods：The effects of the influences such as emulsifiers,emulsification temperature,gelatin/sodium alginate optimum concentration,gelatin and alginic acid sodium ratio,ratio of shikonin to wall materials and Ca Cl2 concentration on the formulation of microcapsule were studied by using L9（34）orthogonal test with standard of encapsulation efficiency,drug loading,visual form and size distribution as optimization index. Results：The optimum preparation formulation of microencapsulation of shikonin was selected as follows：the ratio of 2% gelatin and sodium alginate was 1∶3,the ratio of shikonin and wall material was 1∶1,the concentration of Ca Cl2 was 5%,Tween-Span（1∶1）is selected as emulsifier,the emulsification temperature was 55 ℃. The results indicated that the shikonin microcapsules were prepared with good spherical and uniform particle size distribution under above condition. And the drug loading and encapsulation efficiency were 18.27% and 81.90%,respectively. Conclusion：Preparation of shikonin gelatin-sodium alginate microcapsules shows a good encapsulation efficiency and drug loading as well as uniform particle size,which can provide a foundation for further study of shikonin microcapsules.
作者
梁莉靖
岳贵娟
刘明玉
刘湉
高艳艳
姜悦
陈国超
马群
LIANG Lijing;YUE Guijuan;LIU Mingyu;LIU Tian;GAO Yanyan;JIANG Yue;CHEN Guochao;MA Qun(Beijing University of Chinese Medicine, Beijing 102488, China)
出处
《辽宁中医药大学学报》
CAS
2018年第7期89-92,共4页
Journal of Liaoning University of Traditional Chinese Medicine
关键词
紫草素
微囊
制备工艺
正交实验
shikonin
microcapsule
preparation formulation
orthogonal experiment
