摘要
目的：采用HPLC法测定三七总皂苷分散片中三七皂苷R1、人参皂苷Rb1和Rg1三种皂苷含量，为建立三七总皂苷分散片质量标准含量测定方法提供依据。方法：用Zorbax Eclipse XDB-C18（4．6×250mm，5μm）分析柱；乙腈-水线性梯度洗脱；流速1．0mL／min，检测波长203nm。结果：三七皂苷R1、人参皂苷Rg1和Rb1的线性范围分别为0．12～2．4μg，0．4～8μg，0．5～10μg。该方法回收率三七皂苷R1为99．36％（RSD=1．53％）、人参皂苷Rg1为98．69％（RSD=0．86％）和人参皂苷Rb1为98．92％（RSD=1．27％）。结论：HPLC梯度洗脱能将三种皂苷很好地分离检测，方法准确灵敏，稳定可靠，可用于三七总皂苷分散片的质量控制。
Objective ： Applying HPLC method to study on the determination of Notoginsenoside Rl and Notoginsenoside Rgl, RbI content and providing the proof of quality specification in the dispersible tables of total Notoginsenosides of Panax Notoginseng. Method： The Zorbax Eclipse XDB-C18 column was used with a mobile phase of acetonitrile-water gradient elution, the flow rate was set at 1.0mL/min and the wavelength of detector was set at 203 nm. Results ： The linear range of R1 , Rg1, RbI was 0. 12 - 2.4μg, 0.4 - 8 μg, 0. 5 - 10μg. The average recovery was 99.36% , 98.69% , 98.92%. Conclusion： The method is rapid, accurate, sensitive and reliable. The result showed that the method can be used to control quality of products.
出处
《云南中医学院学报》
2007年第2期4-6,共3页
Journal of Yunnan University of Traditional Chinese Medicine
基金
云南省教育厅资助课题(NO:04Y357C)
关键词
三七总皂苷
分散片
HPLC法
含量测定
Total Notoginsenosides of Panax Notoginseng
Dispersible Tablets
HPLC
Quantitative Determination
