摘要
目的建立三七总皂苷中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量测定方法。方法采用RP-HPLC法,色谱柱采用菲罗门GeminiC18柱(4.6mm×250mm,5μm),流动相A为水;B为乙腈;梯度洗脱条件为:0～5min(23∶77),5～10min(23～25∶77～75),10～35min(25～40∶75～60),35～36min(40～23∶60～77),36～40min(23∶77);检测波长203nm。结果三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1分别在25.03～150.2mg·mL-1,101.6～609.6mg·mL-1,104.7～628.2mg·mL-1范围内线性关系良好,r均为0.9999,方法重复性及回收率均符合要求。结论本法简便、准确、快捷,适用于对三七总皂苷中3种成分含量的同时测定。
Aim A high-performance liquid chromatography （HPLC） method was developed for determination of sanchinoside R1, ginsenoside Rg1, ginsenoside Rb1 in panax notoginseng saponions extracts. Methods RP-HPLC method was employed on ODS column. Sanchinoside R1, ginsenoside Rg1 , ginsenoside Rb1 were eluated with acetonitrile （A）-H20 （B） （A： B,V/V,23：77 from 0 to 5min,23 -25：77 -75 from 5 to 10min, 25 -40：75 -60 from 10 to 35min, 40 -23：60 -77 from 35 to 36rain, 23：77 from 36 to 40min）. Results Sanchinoside R1, ginsenoside Rg1, ginsenoside Rb1 were detected at 203nm. The HPLC method showed a good linear relationship in the range of 25.03 - 150.2mg·L^-1 for sanehinoside R1 , 101.6 -609.6 mg·L^-1 for ginsenoside Rgl and 104.7 -628.2mg·L^-1 for ginsenoside Rb1. Their coefficients of determination （ r ） were 0.999 9. Conclusion This HPLC method was simple, aceurate and instant, which can be used for content determination of panax notoginseng saponions extracts.
出处
《解放军药学学报》
CAS
2009年第1期84-86,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
