摘要
利用不同浓度的水杨酸溶液处理苗龄为7d的大白菜植株,在处理12h后提取粗酶液并测定酶活。分别采用Trizol试剂法、改良SDS法、异硫氰酸胍法等方法对酶活力最高试验组的叶片提取RNA进行RT-PCR反应。结果表明,0.6mmol.L-1水杨酸浓度诱导试验组效果最佳,几丁质酶的活力达到最大值;改良SDS法提取的大白菜叶片总RNA完整性好、纯度高,提取的RNA可用于RT-PCR及RACE等实验操作。
Experiments were conducted on Chinese cabbage seedlings of the seventh day after germination with varied-concentration salicylic acid. Total RNA was prepared from the leaves with higher chitinase activity by methods of Trizol, guanidine isothiocyanate and SDS, and RT-PCR reaction was carried out. The results showed that the chitinase activity reached maximum with 0. 6 mmol · L^-1 salicylic acid about 12 h,and the special band were amplified by SDS extracting, which suggests that the modified SDS method is an efficient and effective procedure for the extraction of high quality and yield RNA from Chinese cabbage.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2007年第2期248-250,共3页
Journal of Anhui Agricultural University
基金
安徽省教育厅自然科学研究项目(2006KJ211B)资助
