摘要
为探讨改良SDS法是否适用于杏基因组DNA提取及提取液适宜浓度,以6个品种杏叶片为试材提取DNA,提取液设0.5%、1%、2%三个浓度梯度。检测结果表明,改良SDS法完全适用于杏叶片基因组DNA的提取,3种浓度的提取液均可得到质量较高的DNA,且0.5%为改良SDS法提取杏叶片基因组DNA的适宜提取液浓度。
To probe the adaptability of modified SDS method in apricot genomic DNA extraction and the optimal concentration, the genomic DNA samples of 6 apricot cultivars were isolated by 0.5%, 1%, 2%SDS extracted buffer. The results showed that good quality DNA would be obtained by modified SDS method and the 0.5% is better.
出处
《中国农学通报》
CSCD
2008年第4期69-71,共3页
Chinese Agricultural Science Bulletin
基金
北京市科技合同项目"干果良种资源的选育与开发"(955422200)
关键词
杏
DNA提取
SDS法
apricot, DNA extraction, SDS method
