摘要
目的:设计操作简单、成本低、便携和易于实现自动化等特点的小型化现场病原体检测系统,实现在传染病疫情爆发现场对传染病病原体的快速检测。方法:设计一款封闭式病原体检测卡盒,可进行一体化病原体核酸检测,并创新性地采用3D打印技术对该卡盒进行迭代设计和制造。在此基础上,研发与之配套的全自动便携式核酸检测系统,整套系统可自动化完成病原体核酸的单重检测或多重检测。结果:在卡盒和全自动便携式核酸检测系统设计完成后,分别对其进行各项性能的测试,如卡盒的气密性和移液性能,混匀性能等,测试结果均能满足病原体核酸检测的要求。以百日咳杆菌为代表,将整套系统应用于实际的病原体核酸检测,实验结果表明该卡盒系统可自动化完成核酸检测。结论:该系统能够成功地完成现场传染病从样本输入到结果输出的自动化一体化检测过程,实验结果准确可靠。
Objective ：To develop a simple,low cost,portable and easy to realize automation pathogen nucleic acid detection system to meet the rapid on-site testing requirements. Methods ：An enclosed cartridge was designed for integrated pathogen nucleic acid testing and creatively manufactured using 3D printing technology that speeded up the development cycle and saved the cost of the whole system. On this basis, a fully automatic portable system was developed,which now is able to automatically and integratedly realize single or multiple nucleic acid detection. Results：Various tests of this instrument were carried out,induding airtightness of car- tridge and pipetting performance ,blending performance, etc. All test results met the requirements of pathogen detection. At last, take pertussiss for example, the practical applications of nucleic acid detection showed that the cartridge system can complete nucleic acid testing automatically. Conclusion：This system can successfully and automatically complete the integration testing process of infectious diseases from sample input to result output. The experimental results are accurate and reliable.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第2期139-148,共10页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(61471168,61527806)
中国博士后科学基金(2016T90403)
经济林培育与利用湖南省高等学校2011协同创新中心[(2013)448]
关键词
封闭式卡盒
现场检测
核酸检测系统
多重PCR
3D打印
enclosed cartridge
on-site detection
nucleic acid detection system
multiple PCR
3D printing
