摘要
本研究通过观察初治与难治急性非淋巴细胞白血病中NF-κB活性及WT1、MDR1的表达水平,探讨三者在急性非淋巴细胞白血病疗效中的作用与相互关系,为寻找新的改善难治急性非淋巴细胞白血病疗效的方法提供理论依据。急性非淋巴性白血病患者45例,其中初治组20例(A组),难治组25例(分为B、C两组)。以15例单纯缺铁性贫血患者作为对照组。采用凝胶电泳迁移分析(EMSA)法检测各组病人NF-κB的活化水平,用逆转录多聚酶链式反应(RT-PCR)法检测各组病人WT1及MDR1的表达水平。结果发现,在对照组未检测到NF-κB活化及WT1和MDR1表达;在初治组中NF-κB的活化水平、WT1和MDR1表达水平均明显低于难治组,而在难治B、C两组中三者无明显区别;各组病人细胞中NF-κB活化水平与WT1及MDR1的表达水平呈正相关。结论:NF-κB的活化,WT1、MDR1的高表达可能是急性非淋巴细胞白血病难治的原因之一,急性非淋巴细胞白血病中NF-κB的活化水平与WT1、MDR1的表达呈正相关。
The study was aimed to explore the NF-κB continual activity and the expression of WT1 and MDR1 in acute non-lymphocytic leukemia （ANLL） patients, and to investigate if the three factors affect the curative effect of ANLL together as to provide some theoretical basis for finding new measures to improve the curative effect of refractory ANLL. The bone marrow samples of 45 ANLL patients was collected. 45 patients including 20 primary ANLL patients （ A group） and 25 refractory ANLL patients. Refractory ANLL patients were divided into 2 sub-groups （B, C groups）. The primary patients who was no effect after more than two courses of treatment were taken as group B, and the patients with more than two relapses were taken as group C. At the same time , 15 patients with simple iron deficiency anemia were collected as negative control. The NF-κB continual activity was measured by using electrophoretic mobility shift assay（EMSA） and the expressions of WT1, MDR1 were detected by reverse transcriptase polymerase chain reaction （RT-PCR）. The results showed that the activity of NF-κB and the expressions of WT1, MDR1 were not detected in 15 samples of simply iron deficiency anemia subjects. The NF-κB continual activity , the expression levels of WT1 and MDR1 in the refractory group were significantly higher than that in primary group （P 〈 0.001 ）. But the NF-κB continual activity, the expression of WT1 gene and MDR1 gene were not significantly different between group B and group C （P 〉 0.05）. By assaying the relativity between the them the NF-κB continual activity and the expression of WT1 or MDR1 had positive correlation in ANLL patients. It is concluded that the NF-κB continual activity, the overexpression of WT1 and MDR1 may be one of the reasons causing poor curative effect in acute non-lymphocytic leukemia. The NF-κB continual activity and the expression of WTI, MDR1, all show positive correlation in ANLL patients.
出处
《中国实验血液学杂志》
CAS
CSCD
2007年第2期253-257,共5页
Journal of Experimental Hematology
