摘要
本研究探讨硼替佐米(bortezomib,Bor)单用或联合三尖杉酯碱(harringtonine,HT)对HL-60细胞凋亡的影响。以不同浓度Bor、HT处理HL-60细胞12-48小时,采用MTT比色法检测细胞增殖活性,以DNA凝胶电泳、荧光显微镜检、流式细胞术检测细胞凋亡。结果表明:10-50nmol/LBor可有效抑制HL-60细胞增殖,并诱导其凋亡。其中10nmol/L剂量处理12小时即有细胞凋亡发生,延长作用时间或增加药物剂量可使细胞凋亡率明显增加。30nmol/LHT与10nmol/LBor联合应用时,可见HL-60细胞凋亡率比两种药物单独使用时显著增加。结论:Bor对HL-60细胞具有时间和剂量依赖性的细胞凋亡诱导效应,与HT联合应用有明显的协同作用。
The aim of this study was to investigate the effect of bortezomib alone and in combination with harringtonine on apoptosis of HL-60 cells. ILL-60 cells were treated with bortezomib, harringtonine in different concentrations for 12 -48 hours. Cell proliferation was analyzed by MTT assay; the apoptosis of HL-60 cells was observed by DNA gel electrophoresis, fluorescence microscopy and flow cytometry. The results showed that 10 -50 nmol/L bortezomib could effectively inhibit HL-60 cell proliferation, and induced its apoptosis. After treating for 12 hours, 10 nmol/L bortezomib could trigger cells apoptosis. With time prolongation or dose increase, HL-60 cell apoptotic rate significantly increased. Furthermore, co-administration of bortezomib （10 nmol/L） with harringtonine （30 nmol/L） resulted in a higher cell apoptotic rate when compared with that induced by those agents used alone. It is concluded that the bortezomib can induce HL-60 cells apoptosis in a time-and-dose-dependent manner and synergistic effectiveness can be found when bortezomib combined with harringtonine.
出处
《中国实验血液学杂志》
CAS
CSCD
2007年第2期233-236,共4页
Journal of Experimental Hematology
基金
广东省科技攻关项目(B30202)
