摘要
目的探讨乳腺癌组织中缺氧诱导因子-1α(HIF-1α)的表达及其与增殖细胞核抗原(PCNA)、微血管密度(MVD)及临床病理因素的关系。方法采用免疫组化SP法检测乳腺纤维腺瘤、普通乳腺增生组织和乳腺癌组织中HIF-1α及PCNA蛋白的表达,用CD34单克隆抗体标记血管内皮细胞并计数MVD。结果HIF-1α蛋白在乳腺纤维腺瘤、普通乳腺增生组织中不表达;在乳腺导管内原位癌中表达阳性率为55.0%(11/20),浸润性乳腺癌中为85.0%(51/60);HIF-1α表达与淋巴结转移及雌激素受体状态有关(P<0.01)。乳腺癌中PCNA高表达率为75.0%(60/80),其中导管内原位癌中为65.0%(13/20),浸润性癌中为78.3%(47/60)。乳腺癌中HIF-1α表达与PCNA呈正相关(r=0.693,P<0.01),与导管内原位癌中MVD亦呈正相关(r=0.682,P<0.05),与浸润性癌中MVD无相关关系。结论HIF-1α在乳腺癌组织中的表达明显上调,与肿瘤细胞增殖、血管形成、淋巴结转移、雌激素受体状态相关,提示HIF-1α对乳腺癌的肿瘤细胞增殖、血管形成、生长和侵袭、转移起重要作用,有望成为肿瘤治疗的新靶点。
Objective To investigate the expression of hypoxia inducing factor 1 alpha （HIF-1α） in human breast cancer and its relationships with microvessel density （MVD）, proliferating cell nuclear antigen （PCNA） protein, other tumor biomarkers and clinicopathologic factors. Methods Immunohistochemical staining （SP） was used to measure the expressions of HIF-1α and PCNA in human breast fibroadenoma, usual hyperplasia and adenocarcinoma, and the MVD was determined by anti-CD34 immunostaining. Results No HIF-1α was observed in the lesions of breast fibroadenoma and hyperplasia. However, the positive expression rate of HIF-1α in the ductal carcinoma in situ （DCIS） was 55.0% （11/20） and the infiltrative breast cancer was 85.0% （51/60）. The total high expression rate of PCNA in breast cancer was 75.0% （60/80）, in which the rate of DCIS counted for 65.0% （13/20） and the rate of infiltrative adenocarcinoma counted for 78.3% （47/60）. There were positive correlations between the expresson of HIF-1α and the expression of PCNA （r=0. 693, P〈0.01） and MVD in DCIS （r=0. 682, P〈0.05）, respectively, but there was no relation between HIF-1α and MVD in infiltrative breast cancer. The expression of HIF-1α was associated with tumor cell proliferation, lymph node metastasis, estrogen receptor status （P〈0.01）. Conclusion The expression of HIF-1α increased in breast cancer and it is associated with tumor cell proliferation, lymph node metastasis, estrogen receptor status. Thus, HIF-1α may play an important role in the tumor cell proliferation, vasiformation, progression and metastasis of breast cancer, and may become a new target for tumor treatment.
出处
《中国普外基础与临床杂志》
CAS
2007年第2期203-207,共5页
Chinese Journal of Bases and Clinics In General Surgery
关键词
乳腺癌
缺氧诱导因子-1Α
增殖细胞核抗原
微血管密度
Breast cancer Hypoxia inducing factor 1 alpha Proliferating cell nuclear antigen Microvessel density
