摘要
目的观察人类铜转运蛋白(human copper transporters 1,hCTR1)、脆性组氨酸三联体(fragile histidine triad,FHIT)和增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)在子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)及子宫颈鳞癌(squamous cell carcinoma,SCC)中的表达,探讨SCC中三指标之间的相关性。方法采用免疫组化SP法检测94例CIN、40例SCC及23例正常宫颈组织中hCTR1、FHIT和PCNA的表达。结果 (1)hCTR1在SCC(87.50%)及CIN 2～3(74.63%)的阳性率均明显高于正常组(17.39%)、CIN 1(29.63%),差异有统计学意义(P<0.001)。(2)FHIT从正常组(100%)、CIN 1(74.07%)、CIN 2～3(44.78%)到SCC(32.50%),FHIT阳性细胞表达逐渐减少。FHIT在各组间阳性率比较差异有统计学意义(P<0.001);FHIT阳性率在SCC及CIN组明显低于正常组中的表达,差异有统计学意义(P<0.001);FHIT阳性率在CIN 2～3与CIN 1之间的表达,差异有统计学意义(P<0.05)。(3)PCNA在CIN 2～3(64.18%)、SCC(75.00%)中阳性表达,明显高于正常组(26.09%),差异有统计学意义(P<0.05,P<0.001);CIN 2～3与CIN 1之间PCNA阳性率差异有统计学意义(P<0.05)。(4)在SCC中FHIT和PCNA表达具有负相关性(P<0.05,r=-0.377);hCTR1与PCNA、FHIT的表达之间均无相关性(P>0.05),r hCTR1与PCNA=0.026,r hCTR1与FHIT=-0.296。结论 (1)hCTR1在子宫颈原位癌阳性细胞比率要高于子宫颈早期浸润癌中hCTR1的表达。提示hCTR1与恶性肿瘤生物学特征侵袭可能存在负相关性。(2)FHIT蛋白和PC-NA共同参与调节细胞增殖,二者存在一种平衡关系。检查子宫颈CIN中的FHIT和PCNA蛋白表达情况,可用作高级别的CIN筛查或预测。
Purpose To study the expression of human copper transporters 1 （hCTR1）, fragile histidine triad （FHIT） and prolifera- ting cell nuclear antigen （PCNA） in cervical intraepithelial neoplasia （CIN） and cervical invasive squamous cell carcinoma （SCC） and investigate their correlativity in SCC. Methods hCTR1, FHIT and PCNA were examined by immunohistochemical staining （ SP method） in 94 cases of CIN, 40 cases of cervical invasive SCC, and 23 cases of normal cervical squamous epithelia. Results The positive rates of hCTR1 in SCC （87.50%） and in the level CIN 2 - 3 （74.63 % ） were significantly higher than those of normal cervi- cal squamous epitheliums （17.39%） and CIN 1 （29.63%） （P 〈 0. 001 ）. In normal cervical squamous epithelium, FHIT positive rate was 100%. From normal cervical squamous epithelium to CIN （ CIN 1, 74.07% , CIN 2 - 3, 44.78% ） and SCC, FHIT ex- pressed cells gradually decreased. The positive rates of FHIT were different between every group （ P 〈 0. 001 ）. The expression of FHIT in the SCC and in CIN were significantly lower than those of normal cervical squamous epithelium （ P 〈 0. 001 ）. The differences be- tween CIN 2 - 3, SCC and CIN 1 were significant （ P 〈 0. 05 ）. The expression of PCNA in SCC （ 75.00% ） and in CIN 2 - 3 （64. 18% ） were significantly higher than those of normal cervical squamous epithelium （26. 09% ） （P 〈 0.05, P 〈 0. 001 ）. The differences between CIN 2 - 3 and CIN 1 were significant （ P 〈 0. 05 ）. There was a positive correlation between expression of FHIT and PCNA （P 〈 0. 05, r = - 0. 377）, but no correlation was found between hCTRI and FHIT （P 〉 0. 05, r = - 0. 296） or hCTR1 and PCNA （P 〉 0. 05, r = 0. 026）. Conclusions hCTR1 expression intensity and positive rate in cervical carcinoma in situ were higher than that in early invasive cancer in cervix, which may prove that there is a negative correlation between hCTR1 expression and malig- nant biological character
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2012年第9期970-974,共5页
Chinese Journal of Clinical and Experimental Pathology
