摘要
观察了三种紫外线(UVA、UVB和UVC)对两种发光细菌(青海弧菌(V.qinghaiensis)和费氏弧菌(V.fischeri))发光的影响.结果表明,三种紫外线均能在数分钟内抑制发光细菌的发光,并随辐照时间的延长而抑制增加,但细菌发光光谱并不改变.其中UVC对发光的抑制作用最强,依次为UVB和UVA.实验表明,极微小能量的紫外辐照(22μW/cm2)6min即足以导致发光被抑制.此外,紫外辐照造成细菌丙二醛含量明显升高,表明紫外辐照后细胞膜的脂质发生过氧化作用,细胞膜受到损伤,进而使细菌发光受到影响.
The influence of three kinds of Ultra-Violets （UVA, UVB and UVC） on two species of luminescent bacteria （V. qinghaiensis and V. fischeri） was studied. The results indicated that all three kinds of Ultra-Violets could inhibit the luminescence of the two species of luminescent bacteria. And with the increase of irradiation time, the inhibiting effects were enhanced without the changing of luminescent spectrum. UVC had the best inhibitory effect, UVB and UVA were in the next place. The experiments showed that even little irradiation（22 μW/cm^2） would cause inhibition of luminescence in a few minutes. Furthermore, the MDA content produced by UV irradiation increased evidently. This phenomenon indicated that UV caused cellular membranes＇ LPO which damaged the cellular membranes and inhibited the luminescence.
出处
《华东师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第2期98-103,共6页
Journal of East China Normal University(Natural Science)
关键词
紫外线
紫外损伤
发光细菌
辐照
生物发光
Ultra-Violet
UV damage
luminescent bacteria
irradiation
bioluminescence
