摘要
【目的】研究非病毒基因载体聚乙二醇(PEG)-聚乙烯亚胺(PEI)共聚物的组成对体外介导基因传递的影响。【方法】将含PEG不同分子量和接枝量的PEG-PEI共聚物,与DNA形成复合物。考察带正电荷的PEI与带负电荷的DNA的相互作用,测定了PEG-PEI/DNA复合物的粒径和Zeta电位,及对Hela细胞的毒性和转染率。【结果】PEG侧链并未明显影响PEI与DNA形成复合物的能力;连接PEG5000能够明显降低复合物的粒径;复合物的Zeta电位随着PEG接枝量的增加而降低;细胞毒性不依赖于PEG的分子量的变化,而是取决于PEG的接枝量;共聚物PEG-PEI(2-25-1)被证实为较有效的介导体外基因传递的复合物。【结论】共聚物的结构组成对DNA复合物的理化性质、毒性和转染率都产生较大的影响。
[Objective] To study the influence of copolymer polyethylene glycol （PEG）- polyethylenimine （PEI） composition on gene delivery in vitro. [Methods] The PEG - PEI /DNA complexes were formed with different molecular weights of PEG and degree of PEGylation. The interaction between the positively charged PEI and the negatively charged plasmid DNA was investigated. The complex size and zeta potential of copolymer/DNA complexes were measured, and the cytotoxicity and the transfection efficiency for Hela cells were evaluated. [Results] The PEG side chains had no significant effect on the complex formation and protection of DNA. PEG 5 k significantly reduced the diameter of the complexes. The zeta potential of complexes reduced with increasing degree of PEG grafting. The cytotoxicity of the complexes did not change with molecular weight of PEG, but changed with the degree of PEG substitution; Copolymer PEG-PEI（2-25-1） was proved to be a better carrier for in vitro gene transfer. [Conclusion] The copolymer block structure strongly influences not only the physicochemical properties of the DNA complexes, but also their cytotoxicity and transfection efficiency.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2007年第2期131-136,共6页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家自然科学基金资助项目(30570500)
