摘要
目的:建立以高效液相色谱法测定小叶黑柴胡茎叶中槲皮素、异鼠李素含量的方法。方法:色谱柱为KromasilC18(250mm×4.6mm,5μm),流动相为甲醇-0.4%磷酸溶液(55∶45),流速为1.0mL.min-1,检测波长为256nm。结果:槲皮素的线性范围为0.08～0.40μg(r=0.9996),平均回收率为101.02%(RSD=1.53%);异鼠李素的线性范围为0.06～0.30μg(r=0.9992),平均回收率为101.26%(RSD=2.95%)。结论:本法操作简便、准确、重现性好,可用于小叶黑柴胡药材的质量控制。
OBJECTIVE： To establish an HPLC method for determining the contents of Quercetin and Isorhamnetin in Bupleurum..smithii var.parvifolium Shah et Y.Li. METHODS： The HPLC was performed on Kromasil column C18 （250mm×4.6mm,5μm）,using methanol- 0.4% phosphoric acid（55 ： 45）as mobile phase,with flow rate at 1.0mL · min^- 1 and detection wavelength at 256nm. RESULTS： The linear range of Quercetin was 0.08-0.40μg（r = 0.999 6）, with an average recovery rate of 101.02% （RSD = 1.53%）; that of Isorhamnetin was 0.06-0.30μg（r = 0.999 2）, with an average recovery rate of 101.26% （RSD = 2.95% ）. CONCLUSION ： This method is simple and accurate with good reproducibility . It is suitable for the quality control of Bupleurum.smithii var.parvifolium Shan et Y. Li.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第9期693-695,共3页
China Pharmacy
关键词
高效液相色谱法
小叶黑柴胡
槲皮素
异鼠李素
含量测定
HPLC
Bupleurum.smithii var.parvifolium Shan et Y.Li
Quercetin
Isorhamnetin
Content determination
