摘要
目的:建立以反相高效液相色谱法测定消炎利尿合剂中槲皮素含量的方法。方法:色谱柱为Syltech-500C18(250mm×4.6mm,5μm),流动相为甲醇-0.2%磷酸(50∶50),流速为1.0mL.min-1,检测波长为360nm,柱温为25℃。结果:槲皮素检测浓度在19.2～96.0μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9994);平均加样回收率为99.40%,RSD=2.31%(n=6)。结论:本方法专属性强、灵敏度高、干扰小、结果可靠,可用于消炎利尿合剂的质量控制。
OBJECTIVE： To establish RP- HPLC for the determination of the content of Quercetin in the Xiaoyanliniao mixture. METHODS： Samples were Separated on Syltech - 500 C18 column（250mm × 4.6mm, 5μm） . The mobile phase con- sisted of methanol - 0.2% phosphoric acid（50 ： 50） at a flow rate of 1.0mL · min^-1.The detection wavelength was 360nm, and the column temperature was 25℃. RESULTS： The linear range of Quercetin was from 19.2 to 96.0μg · mL^-l（r = 0.999 4）. The average recovery was 99.40% （RSD = 2.31%, n = 6）. CONCLUSION： The method was specific, sensitive and reliable in results yet with little interference, and it can be used as quality control for Xiaoyanliniao mixture.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第33期2598-2600,共3页
China Pharmacy
