摘要
目的研究神经生长因子(NGF)对新生鼠缺氧缺血性脑损伤(HIBD)后,神经干细胞巢蛋白(nestin)及血管内皮生长因子(VEGF)表达的影响,探讨其神经修复作用机制。方法7日龄新生SD大鼠84只,建立HIBD模型后,随机分为假手术组、缺氧缺血组及NGF干预组各28只。神经生长因子干预组腹腔内注射NGF(6000U/kg/d),缺氧缺血组同步注射等量生理盐水。采用免疫组化法检测NGF对新生大鼠HIBD后不同时间内Nestin及VEGF表达的影响。结果缺氧缺血性脑损伤后12、24h,3、7、14d,NGF干预组在大脑皮质、海马和室旁区的Nestin表达高于缺氧缺血组(P<0.05),且高峰提前到3d。HIBD后即刻、3、12、24h及3d,NGF干预组在大脑皮质、海马区VEGF的表达高于缺氧缺血组(P<0.05),且高峰提前到3h。结论NGF对HIBD具有神经修复作用可能是通过上调nestin及VEGF的表达而实现的。
Objective To study the effects of nerve growth factor （NGF） on the expressions of Nestin and vascular endothelial growth factor （VEGF） in the brain of the rats with hypoxic-ischemic brain damage （HIBD） and to study the neuro-protective effect of NGF. Methods Eighty four 7-day postnatal SD rats were randomly divided into 3 groups, sham operation （control） group （ n = 28 ）, HIBD group （ n =28） and NGF- treated groups （n = 28）. Immediately after hypoxic-ischemic （HI） insult, 6000U/ （ Kg. d） of NGF or same amount of normal saline was injected intra-peritoneally in NGF-treeded group or HIBD group and control group respectively once a day for 7 days. The expression of Nestin and VEGF in cerebral tissues was tested via immunohistochemical technique. Results The expression of Nestin in NGF-treated group was markedly higher in cortex, hippocampal and periventricular zone at 12 h, 24 h, 3 d and 7 d after HI insult than HIBD group （ P 〈 0.05 ）, and this reached peak at day 3. The expression of VEGF in NGF-treated group increosed in cortex and hippocampal at 0 h, 3 h, 12 h, 24 h, 3 d after HI insult compared loith HIBD group （ P 〈 0.05 ）, and the highest expression appeared at 3 hours. Conclusions The neuro-protective effect of NGF on the newborn rats with HIBD is related to the increased expression of Nestin and VEGF.
出处
《中国新生儿科杂志》
CAS
2007年第2期85-88,共4页
Chinese Journal of Neonatology
关键词
神经生长因子
缺氧缺血
脑
蛋白
Nerve growth factor
Hypoxia-ischemia, brain
Egg white
