摘要
目的:研究小鼠受精卵不同时期中p21蛋白的细胞表达及定位。方法:根据时间点收集不同时期小鼠卵细胞,应用免疫荧光法检测其p21蛋白的细胞定位。同时用Westernblot法检测p21蛋白的表达。结果:在小鼠受精卵G1期p21蛋白分布在胞浆中,S期向胞核聚集,而G2期则集中分布于细胞核中;不同时期小鼠受精卵中p21蛋白的表达无明显差异。结论:p21蛋白在小鼠受精卵发育中的作用与其细胞定位密切相关。
Objective：To investigate expression and location of p21 protein in G1 ,S,and G2 phases of mice fertilized eggs. Methods：The fertilized eggs in G1, S, G2 phases were collected. The expression and location of p21 protein were determined by Western blot and immunofluorescence method,respectively. Results： p21 protein in G1 ,S,and G2 phases of fertilized eggs was located in the cytoplasm,near the nucleus,and in the nuclehs,respectively. No significant difference in p21 expression was found among fertilized eggs in different phases. Conclusion： The location of p21 protein changes in different phases of mouse fertilized eggs.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2007年第1期7-8,共2页
Journal of China Medical University
基金
国家"973"重点基础研究发展规划资助项目(G1999055900-2)
国家自然科学基金重点资助项目(39730460)
