摘要
初步探讨在小鼠受精卵早期发育过程中PKB/Akt对p21蛋白表达及定位的影响。通过显微操作技术注射野生型、持续激活型及激酶失活型的PKB的mRNA,用免疫荧光方法检测p21蛋白的细胞定位、Western blot方法检测p21蛋白的表达。结果显示在注射不同形式的PKB mRNA后p21蛋白的表达无明显差别,但是细胞定位发生改变,PKB被激活后,p21蛋白滞留在胞浆中。因而初步认为在小鼠受精卵中,PKB/Akt通过影响p21的细胞定位而影响细胞周期的进程。
To investigate the effect of Protein kinase B on the expression and location of p21 in mouse early development. Immunopreciptation technology was used to detect the localization of p21 and Western blotting was used to analyze the expression of p21 after micminjecting mRNA of WT-PKB,myt-PKB and PKB-KD to mouse eggs. Them was no obvious difference between the three kinds of mRNA in the expression of p21. But the cell localization altered. The p21 retain in cytoplasm after micmjecting myt-PKB. In mouse fertilized egg PKB/Akt controls the cell cycle by changing the cell localization of p21.
出处
《生物工程学报》
CAS
CSCD
北大核心
2007年第3期493-496,共4页
Chinese Journal of Biotechnology
基金
国家自然科学基金资助项目(No.39730460)
国家重点基础研究发展规划项目(973)(No.G1999055900-2)
辽宁省博士启动基金(No.20041043)资助。~~
