摘要
目的:从中药猪苓中提取猪苓多糖,对其进行硫酸酯化,并比较是否加入分散剂对酯化结果的影响。方法:将纯化的多糖运用尺寸排除色谱和激光光散射联用仪(SEC-LLS)测定其分子量,氯磺酸-吡啶法进行硫酸酯化。通过傅立叶变红外光谱分析酯化前后的结构。结果:得到分子量分布较窄的多糖组分,是一种水溶性的粘多糖,其重均分子量在1.6×105Del左右;并通过硫酸酯化得到硫酸多糖。结论:得到分子量在1.6×105Del左右的β-D-葡萄吡喃糖苷,加入分散剂对酯化收率有很大提高,但会降低其分子量与硫酸基的含量。
Objective： To prepare and sulfate of polysaccharide from Polyporus umbellatus, and to compare the results of joined dispersant or not. Methods ： After purified, SEC-LLS was used to determine the molecular weight of polysaccharide. The polysaccharide was sulfated with the method of chlorosulfonic acid pyridine. The structures of polysaccharide and sulfated polysaccharides was determihated by FI RD analyse. Results ： The results showed that the distribute of molecular weight was narrow. It was a water-soluble glycosaminoglycan with a molecular weight of nearly 1.6 × 10^5 Del. And sulfated polysaccharide was received by sulfation. Conclusion： A kind of f3-D-glucopyranoside was obtained with a molecular weight of nearly 1.6 × 10^5 Del. After added dispersant, the production of sulfated oolysaccharide increased, but the molecular weigh and the content of sulfate group was reduced.
出处
《中药材》
CAS
CSCD
北大核心
2007年第2期160-163,共4页
Journal of Chinese Medicinal Materials
关键词
猪苓多糖
猪苓多糖硫酸酯
硫酸酯化分析
Polyporus umbellatus polysaccharides
Sulfated polysaccharides
Sulfation analysis
