摘要
本文研究了从铜藻中分离提取褐藻糖胶、褐藻淀粉和褐藻胶的方法。用稀盐酸溶液加温搅拌提取藻粉后,在提取液中含有褐藻糖胶和褐藻淀粉,可加乙醇至50％和80％分别将其沉淀出来。藻渣含有褐藻酸钠,可加温加碱而制备出。并用正交实验设计求出提取条件中的温度、pH 和时间的最适配比。对于褐藻糖胶的提取,以温度70℃、时间2小时和提取液pH为4 时最适。对于褐藻淀粉的提取,以温度 70℃、时间 1小时和提取液的 pH为 2时较优。 本文还对粗褐藻糖胶和褐藻淀粉的提纯进行了研究,证明二者均可用酒精分级沉淀法处理而得到纯品,不需要采用较复杂的季胺盐法和离子交换树脂法。 本工作证实铜藻是我国褐藻中含有三种多糖的较好藻种,从中可提褐藻糖胶 3.5％,褐藻淀粉 3.1％和褐藻酸钠 17.8％。
Sargassum, one of the most prevalent seaweeds in China, is also the only onewith a high content of fucoidan, laminaran and algin among all the marine algae inChina. Therefore, the utilization of the seawed is obviously important for our country.In order to furnish some basic data to the seaweed industries, methods for the extrac-tion and isolation of mucilage have been studied.The samples were collected in Huilai Xian, South China. The algae was analysedon dry basis it contains 5.1% of laminaran, 6.1% of fucoidan and 21% of alginicacid.Methods of extraction and isolation: Dried seaweed was extracted with a dilute HClsolution at a constant temperature for a definite time. The extract was then filtratedand washed. Crude fucoidan was precipitated at 50% alcohol. Crude laminaran wasobtained at 80% alcohol. Alginate was extracted from the residue with alkali.The optimum conditions for extraction, consists of stirring for two hours at 70℃with HCl solution at pH 4.Purification: By dissolving one part of crude fucoidan in ten parts of distilledwater and then one part of 4M CaCl_2 solution was added. After centrifugalized, thesupernatant was fractionally preciptated with 30% and 60% of alcohol pecipitation in30% alcohol was impurilles nd in the 60% fraction was fucoidan.On part of crude laminaran was dissolved in five parts of water, and fractionallyprecipitated in 50% and 80% alcohol and in the 80% fraction was Iaminaran.The yields are fucoidan (containing 44. 7% of fucose) 3.9%; laminaran (contain-ing 91% of glucos) 3.1% and sodium alginate 17.8%.
出处
《水产学报》
CAS
1985年第1期71-77,共7页
Journal of Fisheries of China
