摘要
目的建立复方氧氟沙星酮替芬鼻用喷雾剂的含量测定方法。方法采用Beckman ODS柱（4．6min×25cm，5μm），以乙腈-乙酸铵缓冲液为流动相，流速1．0mL·min^-1，检测波长263nm。结果氧氟沙星在30～300mg·L^-1，富马酸酮替芬在20～200mg·L^-1，盐酸麻黄素在100～1000mg·L^-1，羟苯乙酯在3～30mg·L^-1线性关系良好。平均回收率氧氟沙星为98．14％，RSD=0．94％，富马酸酮替芬为98.33％，RSD=0．52％，盐酸麻黄素为98．83％，RSD=1．22％，羟苯乙酯98．91％，RSD=1．87％。结论本方法准确、快捷，适用于该制剂的含量测定。
OBJECTIVE To establish a HPLC method for the simultaneous assaying of compound ofloxacin and ketotifen nasal sprays. METHODS The chromatographic column was Beckman ODS （4.6mm × 25cm, 5μm）; the mobile phase was composed of acetonitrile-ammonium acetate （1 %）with detection wavelength at 263nm and flow speed at 1.0mLmg·L^-1. RESULTS The line range and Correlation coefficient of the ofloxacin were 30-300mg·L^-1,r=0. 999 9; and ketotifen fumarate were 20-200 mg·L^-1, r=0. 999 8; and ephedrine hydrochloride were 100-1000 mg·L^-1, r= 0. 999 8; and ethylparaben were 3-30mg·L^-1,r= 0. 999 6 98.33% 98.91% this nasa The average recoveries ofofloxacin were 98.14%, RSD = 0.94%; and ketotifen fumarate were RSD = 0.52%; and ephedrine hydrochloride were 98.83% RSD = 1.22%; and ethylparaben were RSD= 1.87%. CONCLUSION This methods accurate sensitive and fast. This methods is good at sprays.
出处
《海峡药学》
2007年第2期35-37,共3页
Strait Pharmaceutical Journal
关键词
氧氟沙星
富马酸酮替芬
盐酸麻黄碱
羟苯乙酯
高效液相色谱法
鼻用喷雾剂
：ofloxacin
ketotifen fumarate
ephedrine hydrochloride
ethylparaben
high-performance liquid chromatography
nasal sprays[HJ] [HT]
