摘要
目的建立高效液相色谱-蒸发光散射检测器测定齐墩果酸片含量。方法采用Luna C18（4．6×250mm，5μm）色谱柱，柱温30℃；乙腈-水（88：12）为流动相，流速：1．0mL／min；蒸发光散射检测器检测，漂移管温度90℃，氮气流速：2．30L／min。结果齐墩果酸在0．21～4．16μg之间线性关系良好，r=0．9997（n=8）；回收率为99．24％，RSD=0．44％（n=5）。结论本法灵敏、简便、准确，重线性好。
OBJECTIVE To establish an HPLC-ELSD method for determination of oleanolic acid Tabhs. METHODS With a Luna C18 column（4.6mm × 250mm ,5 μm） at 30℃ ,acetontrile-water（88： 12） was used as the mobile phase,the flow rate was 1.0mL/ min. An evaporative light-scattering detector （ESLD） was used as detector with gas flow rate of 2.30L/min and drift tube temperature of 90℃. RESULTS The oleanolic acid had a good linearity relationship,r =0.999 7 （n = 8）, between 0.21 - 4.16μg. The average recovery was 99.24% with RSD = 0.44% （ n = 5）. CONCLUSION It was proved that the method was reliable,simple, accurate and reproducible.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2007年第1期62-64,共3页
Chinese Journal of Modern Applied Pharmacy
