摘要
目的从细胞增殖和凋亡两方面观察吡罗昔康声动力效应对乳腺癌细胞MDA-MB-231的迟发抑制作用。方法用MTT法检测吡罗昔康声动力作用后MDA-MB-231细胞的增殖能力,采用TUNEL法检测凋亡。结果吡罗昔康声动力效应和单纯超声作用均能明显降低MDA-MB-231细胞的增殖代谢活性而抑制其增殖,但是前者作用更为显著(P<0.01)。TUNEL法染色显示吡罗昔康声动力组和单纯超声组均有数量不等的凋亡细胞,并以前者为著。结论吡罗昔康介导的声动力作用不仅能显著抑制MDA-MB-231细胞增殖,还能有效诱导其凋亡。
Objective To observe the effect of SDT with piroxicam on proliferation and apoptosis of MDA-MB-231 cells. Methods The proliferation inhibitory effect of SDT with piroxicam on MDA-MB-231 cells was detected by MTT colormetric assay; MDA-MB-231 cell apoptosis was observed by TUNEL method. Results SDT with piroxicam and ultrasonic irradiation both obviously inhibited MDA-MB-231 cells proliferation. Compared with the ultrasound irradiation group, the inhibitory effect of the SDT group was significantly higher （ P 〈 0.01 ）. While piroxicam at a dose of 0.2 mmol/L had little influence on the growth of MDA-MB-231 ceils. SDT with piroxicam and ultrasonic irradiation could both induced MDA-MB-231 cell apoptosis, but the SDT group was more obvious. There were few apoptosis cells in the control group. Conclusion SDT with piroxicam not only significantly inhibit the proliferation of MDAMB-231 cells but also induce their apoptosis.
出处
《临床超声医学杂志》
2007年第2期65-67,共3页
Journal of Clinical Ultrasound in Medicine
基金
国家自然科学基金重点(30430230)
面上项目(30370402)
重庆市重点自然科学基金项目(CSTC
2005BA5024)
重庆市科委课题(渝科发计字[2005]34号)
