摘要
以氧合血红蛋白为原料，分别用酸性丙酮萃取—减压蒸馏和酸性丙酮萃取—ＮａＡｃ沉淀两种方法制备氯化血红素。产品用吡啶—ＮａＣｌ的醋酸饱和溶液重结晶进行纯化，通过元素分析，红外光谱和快原子轰击质谱对血红素进行确证。发现粗品中存在着与血红素铁配位的小分子（离子或基团）杂质。这些杂质可以通过重结晶的方法除去。最后还对血红素的质谱裂解机理做了探讨。
Heme is an important kind of natural porphyrin.Thus,it is very sinificant to modify hemeand investigate catalytic and pharmacological activities of the derivatives thus obtained。In thiswork,hemin was prepared fromhemoglobin by two different methods,purified byrecrystallization in pyridin-Nacl- CH_3COOH solution,and identified by element analysisIR-spectrum and fast bombardment mass spectrum(FAB-MS).Agood hemin yelid withhigh purity could be obtained in this preparation and the impurities bonded to theporphyrinoiron were removed by recrystailization。An explanation for the fragment ions of thepositive ion spectrum was given with reference to FAB splitting rules of organic compounds。
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
1995年第3期67-72,共6页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
国务院侨办资助课题
南京大学配位化学国家重点实验室资助
关键词
氯化血红素
血红素
金属卟啉
医学
催化
制备
hemin
derivative or heme
metalloporphyrins
FAB-MS
spliting mechanics
