摘要
目的:探讨上皮钙黏蛋白在宫颈癌中的表达及其与肿瘤发生发展和侵袭转移的关系。方法:采用逆转录-聚合酶链反应(RT-PCR)法检测15例正常宫颈、46例宫颈癌组织中上皮钙黏蛋白mRNA的表达。ELISA检测25例宫颈癌初发患者、25例治疗后患者、25例健康人血清可溶性钙黏蛋白的含量。结果:①上皮钙黏蛋白mRNA表达率宫颈癌明显低于正常宫颈组织(P<0.05);②上皮钙黏蛋白mRNA阳性表达率与患者年龄、生长方式、病理分期无关,与肿瘤的分化程度有关(P<0.05);③初发患者血清可溶性上皮钙黏蛋白显著高于健康人水平(P<0.05),治疗后患者血清水平与初治患者、健康人水平无明显差异。结论:上皮钙黏蛋白低表达或缺失能促进宫颈癌的发生发展,血清可溶性上皮钙黏蛋白的检测可能为肿瘤的早期诊断提供新的手段。
Objective：To study the E - cadherin （E -cad） mRNA expression in cervix cancer, and explore its relations with occurrence, development and metastasis of cancer. Methods： E - cad mRNA expression in cervical tissue was detected by RT - PCR on 46 cases of cervix cancer and 15 healthy controls. The concentration of serum soluble E - cad was determined by ELISA on 25 cases of early cervix cancer, 25 cases of treated cervix cancer and 25 healthy controls. Results： In cancerous cervical tissue, E - cad expression was remarkably lower than that in normal tissue （P 〈 0.05 ） and it tended to decrease with cancer progression （ P 〈 0.05 ）. E - cad expression was associated with the histological grade of cervix cancer （P 〈 0.05）, but not with the patients＇age, clinical stage or growth pattern. The content of serum soluble E -cad in early cervix cancer was significantly higher than that in healthy controls （P 〈 0.05 ）. Conclusion： Reduced E -cad expression or even absence of it may play some role in the occurrence and development of cervix cancer. Thus, detection of serum soluble E - cad concentration may be helpful in early diagnosis of tumor.
出处
《西北国防医学杂志》
CAS
2007年第1期23-25,共3页
Medical Journal of National Defending Forces in Northwest China
基金
陕西省科技攻关基金资助项目(2003K10-G42)
