摘要
目的评价矢状轴位磁共振成像(MRI)胼胝体面积测量和脑脊液 p-tau 蛋白及 Aβ_(1-42)含量对阿尔茨海默病(AD)早期诊断的价值。方法根据 NINCDS-ADRDA 标准诊断的25例可能AD,按 MMSE 评分分为2组:平均 MMSE 评分 AD1组为20.5±2.5;AD2组均为14.7±3,8。另设健康对照组,平均 MMSE 评分27.8±1.7。自动测量软件在 MRI 正中矢状位上对胼胝体面积直接测量。酶标记免疫吸附测定(ELISA)法对各组脑脊液中199位点磷酸化微管相关蛋白(p-tau199)和β-淀粉样蛋白1-42片段(Aβ_(1-42))含量测定。结果 AD1组的脑脊液中 p-tau199(pg/ml)为25.8±3.1,较对照组(17.7±1.5)增高,Aβ_(1-42)(pg/ml)为329.3±15.2,较对照组(522.5±15.1)降低,胼胝体面积较对照组缩小,但程度不显著。AD2组的胼胝体总面积较对照组明显缩小,以整个胼胝体缩小为特点,但尾部改变相对较轻;AD2组与 AD1组比较,p-tau 蛋白含量升高和 Aβ_(1-42)含量(pg/ml)降低的程度更明显,分别为31.3±4.8和318.5±30.8。结论胼胝体面积测量同时配合脑脊液 p-tau 蛋白和 Aβ_(1-42)含量的测定,不仅对确诊早期 AD 有帮助,而且能反映 AD 的病程变化。
Objective Study on the value of the corpus callosum measurement at mid-sagittal slice of magnetic resonance imaging （MRI） and the phosphorylated tan protein （p-tau） and Amyloid beta （Abeta） 1-42 immunoreactive deposits （Aβ1-42 ）assay in cerebral spinal fluid （CSF） on early Alzheimer＇s disease （AD） diagnosis. Methods Consecutive probable AD diagnosed with the National institute of neurology and communication disorder and stroke- Alzheimer disease or AD related disease academy （NINCDS-ADRDA） criteria were divided into two groups according to MMSE score. The average MMSE score were 20. 5 ± 2.5 in group AD1 and 14. 7 ± 3. 8 in Group AD2, and was 27. 8 ± 1.7 in control group, which was well matched with the two AD groups in gender, age and education degree. The area of corpus callosum was measured directly by the autmeas soft ware on MRI, and the levels of p-tau and A151.42 in CSF were assaied by enzyme-labeled immunosorbent assay （ELISA）. Results the levels of p-tau in CSF in AD1 group was（25. 8 ± 3. 1 ） pg/ml, which was higher than that of sex- and age-matched normal control （ 17.7 ± 1.5） pg/ml, and the level of A151.42 in AD1 group was （329.3 ± 15.2） pg/ml, which was lower than that of normal control （522. 5 ± 15.1） pg/ml. AD1 group has a mild corpus callosum shrink. There was a remarkably corpus callosum shrinke in AD2 group, especially at the area of the head and body of callus. The level of p-tau increased and the level of Aβ1-42 decreased remarkably in AD2 group （31.3 ± 4. 8） pg/ml and （318.5 ± 30.8） pg/ml than that in AD1 group. Conclusions The corpus callosum measurement combined with the p-tau and Aβ1-42 assay was useful in early AD diagnosis. Moreover, it was helpful to evaluate AD proceeding.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2007年第1期23-26,共4页
Chinese Journal of Neurology
基金
国家自然科学基金(30370494)
国家博士后基金(2004036272)
